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MilliporeSigma

MAC4L

MetaPolyzyme

lyophilized powder

Synonym(s):

Multilytic Enzyme Mix

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5 MG

$313.00

$313.00

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About This Item

NACRES:
NA.54
UNSPSC Code:
12352204

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form

lyophilized powder

technique(s)

DNA extraction: suitable

suitability

suitable for microbiology

application(s)

microbiology

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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This Item
MAC4LDF10065A4464
MetaPolyzyme lyophilized powder

Sigma-Aldrich

MAC4L

MetaPolyzyme

MetaPolyzyme, DNA free Suitable for Microbiome research, lyophilized powder

Sigma-Aldrich

MAC4LDF

MetaPolyzyme, DNA free

α-Amylase from Aspergillus oryzae powder, ~30 U/mg

Sigma-Aldrich

10065

α-Amylase from Aspergillus oryzae

Enhanced Avian Reverse Transcriptase [eAMV™ RT] For reverse transcription at higher temperatures & rare mRNAs

Sigma-Aldrich

A4464

Enhanced Avian Reverse Transcriptase [eAMV™ RT]

technique(s)

DNA extraction: suitable

technique(s)

DNA extraction: suitable

technique(s)

-

technique(s)

RT-PCR: suitable

application(s)

microbiology

application(s)

microbiology

application(s)

-

application(s)

-

form

lyophilized powder

form

lyophilized powder

form

powder

form

crystalline powder

suitability

suitable for microbiology

suitability

suitable for microbiology

suitability

-

suitability

-

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.

Application

MetaPolyzyme is a mixture of 6 enzymes that is intended for isolation of total DNA for metagenomics studies. Adapted from an initial formulation devised by S. Tighe,2 MetaPolyzyme was evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG).[1] Test data on the use of MetaPolyzyme is accessible at the 2017 ABRF MGRG Poster.

Biochem/physiol Actions

Microbes can be difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted by using lysing enzymes (such as lyticase or chitinase) to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. MetaPolyzyme is intended for use in metagenomic studies, such as study of microbes and microbiomes of extreme and unique environments. MetaPolyzyme is intended for digestion of microbes for evaluation by whole genome shotgun sequencing for metagenomics and metatranscriptomic approaches.

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
The enzymes in MetaPolyzme are:
  • Achromopeptidase
  • Chitinase
  • Lyticase
  • Lysostaphin
  • Lysozyme
  • Mutanolysin

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM7444
SLCK0452
SLCH4841
SLCD6707
SLCD4398

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Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children.
Al-Hebshi NN et al.
Journal of Oral Microbiology, 11(1), 1557986-1557986 (2018)
Microbiome, 4(1), 24-24 (2016-06-04)
The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium is a novel, interdisciplinary initiative comprised of experts across many fields, including genomics, data analysis, engineering, public health, and architecture. The ultimate goal of the MetaSUB Consortium
The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium inaugural meeting report.
MetaSUB International Consortium
Microbiome, 4(1), 24-24 (2016)
Genomic Methods and Microbiological Technologies for Profiling Novel and Extreme Environments for the Extreme Microbiome Project (XMP).
Tighe S et al.
Journal of biomolecular techniques : JBT, 28(1), 31-39 (2017)
Cartography of Opportunistic Pathogens and Antibiotic Resistance Genes in a Tertiary Hospital Environment.
Chng KR et al.
Nature Medicine, 26(6), 941-951 (2020)
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Product Information Sheet - MAC4L

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