The protocol is published in the product datasheet, available in the 'DOCUMENTATION' section of this page, under the 'Mor Documents' tab. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf
MINI26
PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonym(s):
Red PKH membrane label kit
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Available to ship TODAYfromMILWAUKEE | $324.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
$324.00
Available to ship TODAYDetails
Subscriptions are currently unavailable.
Ships Every 4 weeks
packaging
pkg of 1 kit
Quality Level
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable
fluorescence
λex 551 nm; λem 567 nm (PKH26 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
General description
This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.
Application
PKH26 red fluorescent cell linker mini kit has been used for:
- labelling microglial, natural killer cells and preadipocytes
- tracking of viable transplanted adipose-derived stem cells
- in vitro proliferation studies of bone marrow stem cells
- general cell membrane labeling, including for in vitro cell labeling and long term in vivo cell tracking.
Other Notes
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
CellVue is a registered trademark of Phanos Technologies
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
| manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename - |
Kit Components Only
Product No.
Description
- Diluent C 10 mL
- PKH26 Cell Linker in ethanol .1 mL
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
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Articles
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.
Transplanted bone marrow stem cells relocate to infarct penumbra and co-express endogenous proliferative and immature neuronal markers in a mouse model of ischemic cerebral stroke
Zhang XM, et al.
BMC Neuroscience, 11(1), 138-138 (2010)
Human leukocyte antigen-G1 inhibits natural killer cytotoxicity through blocking the activating signal transduction pathway and formation of activating immunologic synapse
Yu Y, et al.
Human immunology, 69(1), 16-23 (2008)
Adipose tissue regeneration in vivo using micronized acellular allogenic dermis as an injectable scaffold
Lee HY, et al.
Aesthetic Plastic Surgery, 38(5), 1001-1010 (2014)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MINI26-1KT | 04061834066712 |
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MINI26-1KTの実験プロトコルを入手したいのですが、どこにありますか？
1 answer-
Helpful?
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Is it possible to lable cells with PKH26 at 4oC?
1 answer-
The membrane dyes will remain stable during the monoclonal staining at 4 deg C; however, capping of the monoclonal antibodies is highly probable if the general cell membrane labeling is carried out at ambient temperature subsequent to antibody labeling. As additional information may be required to address the question completely, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the information needed so that a member of the Technical Service team can reach out to assist further.
For more information, please refer to the product data-sheet below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf.
Helpful?
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