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N2133

Neuraminidase from Clostridium perfringens (C. welchii)

Type X, lyophilized powder, ≥50 units/mg protein (using 4MU-NANA)

Synonym(s):

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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1 unit
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$255.00
5 units
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$955.00
10 units
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$1,550.00
50 units
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$5,460.00

About This Item

CAS Number:
9001-67-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-624-6
MDL number:
MFCD00131711
EC Number:
3.2.1.18 (BRENDA, IUBMB)
Specific activity:
≥50 units/mg protein (using 4MU-NANA)

$255.00

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type

Type X

Quality Segment

form

lyophilized powder

specific activity

≥50 units/mg protein (using 4MU-NANA)

storage temp.

−20°C

Gene Information

Clostridium perfringens str. 13 ... nanI(988807)

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Clostridium perfringens has been used in a study to assess purification via affinity chromatography. It has also been used in a study to investigate site-directed mutations of amino acids of the neuraminidase gene, nanH.

Biochem/physiol Actions

Neuraminidase can block attachment of type 3 reovirus to cell membranes. This effect is related to the ability of neuraminidase to hydrolysis sialic acid residues within cell surface receptors.
Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.
Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.
The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.

Preparation Note

Purified by affinity chromatography from Type VIII (N 5631)

Analysis Note

Package sizes based on 4MU-NANA units
Package sizes based on the 4MU-NANA units

Other Notes

One unit will hydrolyze 1.0 micromole of 2′-(4-Methylumbelliferyl)-alpha-D-N-actetylneuraminic acid per min at pH 5.0 at 37 °C.

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This Item
N5631N3001N2876
Neuraminidase from Clostridium perfringens (C. welchii) Type X, lyophilized powder, ≥50 units/mg protein (using 4MU-NANA)

Sigma-Aldrich

N2133

Neuraminidase from Clostridium perfringens (C. welchii)

Neuraminidase from Clostridium perfringens (C. welchii) Type VIII, lyophilized powder, ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin)

Sigma-Aldrich

N5631

Neuraminidase from Clostridium perfringens (C. welchii)

Neuraminidase from Clostridium perfringens (C. welchii) Type VI, lyophilized powder, ≥6 units/mg protein (using 4MU-NANA), ≥2 units/mg protein (mucin)

Sigma-Aldrich

N3001

Neuraminidase from Clostridium perfringens (C. welchii)

Neuraminidase from Clostridium perfringens (C. welchii) Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder

Sigma-Aldrich

N2876

Neuraminidase from Clostridium perfringens (C. welchii)

description

Type X, lyophilized powder, ≥50 units/mg protein (using 4MU-NANA)

description

Type VIII, lyophilized powder, ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin)

description

Type VI, lyophilized powder, ≥6 units/mg protein (using 4MU-NANA), ≥2 units/mg protein (mucin)

description

Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder

specific activity

≥50 units/mg protein (using 4MU-NANA)

specific activity

≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin)

specific activity

≥2 units/mg protein (mucin), ≥6 units/mg protein (using 4MU-NANA)

specific activity

≥0.1 units/mg solid (using mucin), ≥1.3 units/mg solid (using 4MU-NANA)

Gene Information

Clostridium perfringens str. 13 ... nanI(988807)

Gene Information

Clostridium perfringens str. 13 ... nanI(988807)

Gene Information

Clostridium perfringens str. 13 ... nanI(988807)

Gene Information

Clostridium perfringens str. 13 ... nanI(988807)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

type

Type X

type

Type VIII

type

Type VI

type

Type V

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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