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N3393

Naphthol Blue Black

BioReagent, suitable for electrophoresis

Synonym(s):

Acid Black 1, Amido Black 10B, Buffalo Black NBR

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Size/SKUAvailabilityPrice
25 g

Available to ship TODAY

$60.50
100 g

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$164.00

About This Item

Empirical Formula (Hill Notation):
C22H14N6Na2O9S2
CAS Number:
Molecular Weight:
616.49
EC Number:
213-903-1
UNSPSC Code:
41105322
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5374263
Colour Index Number:
20470

$60.50


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product line

BioReagent

Quality Segment

form

powder

composition

Dye content, ~85%

technique(s)

protein electrophoresis: suitable, protein staining: suitable

impurities

≤10% water

suitability

suitable for electrophoresis

SMILES string

[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

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This Item
195243A8181C1144
description

BioReagent, suitable for electrophoresis

description

Dye content 80 %

description

electrophoresis reagent

description

-

technique(s)

protein staining: suitable

technique(s)

-

technique(s)

-

technique(s)

-

suitability

suitable for electrophoresis

suitability

-

suitability

-

suitability

-

form

powder

form

powder

form

liquid

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Dye content, ~85%

composition

Dye content, 80%

composition

-

composition

Dye content, ≥45% (based on Nitrogen)

product line

BioReagent

product line

-

product line

-

product line

-


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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Codes

Hazard Classifications

Skin Sens. 1B

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves



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