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NA0600

GenElute HP Endotoxin-Free Plasmid Megaprep Kit

1 kit sufficient for 5 preparations

greener alternative

Greener Alternative

Synonym(s):

Gen Elute, GenElute Endotoxin-Free Plasmid Kit

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Size/SKUAvailabilityPrice
1 kit

Available to ship TODAYfromMILWAUKEE

$571.00
$513.90

About This Item

NACRES:
NA.52
UNSPSC Code:
41105501

$513.90

List Price$571.00Save 10%
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Quality Segment

usage

 kit sufficient for 5 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

test parameters

sample volume: 200-1000 mL bacterial culture

greener alternative category

storage temp.

15-25°C

General description

The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.
High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Features and Benefits

  • Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
  • Only 90 minutes from pelleted cells to purified plasmid
  • Vacuum format with no ethanol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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This Item
NA0410PLED35PLEX15
description

1 kit sufficient for 5 preparations

description

sufficient for 25 preparations

description

sufficient for 35 purifications

description

sufficient for 15 purifications

usage

 kit sufficient for 5 preparations

usage

sufficient for 25 preparations

usage

sufficient for 35 purifications

usage

sufficient for 15 purifications

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

test parameters

sample volume: 200-1000 mL bacterial culture

test parameters

sample volume: 150 mL bacterial culture

test parameters

: <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture

test parameters

sample volume: 25-200 mL bacterial culture, : <2 hr kit run time for up to 12 preps

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • GenElute HP Pro Megaprep Binding Columns 5
    SDS

  • Column Preparation Solution 2
    SDS

  • Neutralization Solution EF
    SDS

  • Lysis Solution
    SDS

  • E-Toxate Water, endotoxin, free
    SDS

  • Wash Solution 2
    SDS

  • Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )
    SDS

See All (11)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

Target Organs

Central nervous system, Lungs

Storage Class

3 - Flammable liquids

Flash Point (°F)

77.0 °F - closed cup

Flash Point (°C)

25 °C - closed cup

WGK

WGK 3



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