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Anti-p53 antibody produced in sheep

Name: Anti-p53 antibody produced in sheep
Brand: SIGMA
Price: 978 USD

fractionated antiserum, buffered aqueous solution

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Size/SKU	Availability	Price
250 μg	Check Cart for Availability	$978.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164709

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (p), IP, WB

Citations:

$978.00

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Properties

biological source

sheep

Quality Segment

200

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 53 kDa

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: 1-10 μg/mL

UniProt accession no.

P04637

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)
mouse ... Trp53(22059)
rat ... Tp53(24842)

Description

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

GST fusion protein of human p53 corresponding to amino acids 1-393.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	P5813	SAB4503000	SAB4503021
$Anti-p53 antibody produced in sheep fractionated antiserum, buffered aqueous solution$ Sigma-Aldrich P4235 Anti-p53 antibody produced in sheep	Sigma-Aldrich P5813 Anti-p53 antibody, Mouse monoclonal	Sigma-Aldrich SAB4503000 Anti-p53 antibody produced in rabbit	Sigma-Aldrich SAB4503021 Anti-p53 antibody produced in rabbit
description fractionated antiserum, buffered aqueous solution	description clone BP53-12, ascites fluid	description affinity isolated antibody	description affinity isolated antibody
biological source sheep	biological source mouse	biological source rabbit	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form fractionated antiserum	antibody form ascites fluid	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: 1-10 μg/mL	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable, immunocytochemistry: suitable, microarray: suitable, western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein, immunoprecipitation (IP): suitable	technique(s) ELISA: 1:10000, western blot: 1:500-1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100	technique(s) western blot: 1:500-1:1000, immunohistochemistry: 1:50-1:100, ELISA: 1:20000, immunofluorescence: 1:100-1:500
shipped in dry ice	shipped in dry ice	shipped in wet ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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