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P4252

Phosphatase, Alkaline from Escherichia coli

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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100 units

Available to ship TODAYfromMILWAUKEE

$436.00
200 units

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$745.00
1000 units

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$2,090.00
$1,776.50

About This Item

CAS Number:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
MFCD00131849
EC Number:
3.1.3.1 (BRENDA, IUBMB)
Specific activity:
30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Biological source:
Escherichia coli

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biological source

Escherichia coli

Quality Segment

300

form

ammonium sulfate suspension

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

storage temp.

2-8°C

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.

Biochem/physiol Actions

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Suspension in 2.5 M (NH4)2SO4

Analysis Note

The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.

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Phosphatase, Alkaline from Escherichia coli ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

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description

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

description

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

description

buffered aqueous solution, ≥2,000 DEA units/mg protein

description

≥5,500 DEA units/mg protein

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

specific activity

20-50 units/mg protein (in glycine buffer)

specific activity

≥2,000 DEA units/mg protein

specific activity

≥5,500 DEA units/mg protein

biological source

Escherichia coli

biological source

Escherichia coli

biological source

bovine intestinal mucosa

biological source

-

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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