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P4439

Phosphatase, Alkaline from porcine kidney

lyophilized powder, ≥100 DEA units/mg protein

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Size/SKUAvailabilityPrice
100 units

Available to ship TODAYfromMILWAUKEE

$80.10

About This Item

CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
≥100 DEA units/mg protein

$80.10


Available to ship TODAYDetails


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form

lyophilized powder

Quality Segment

specific activity

≥100 DEA units/mg protein

composition

Protein, ≥50%

technique(s)

protein detection: suitable

storage temp.

−20°C

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

Packaging

Package size based on DEA units

Physical form

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

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A2237P8361P5931
description

lyophilized powder, ≥100 DEA units/mg protein

description

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

description

recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein

description

lyophilized powder, 30-60 units/mg protein (in glycine buffer)

technique(s)

protein detection: suitable

technique(s)

protein detection: suitable

technique(s)

protein detection: suitable

technique(s)

protein detection: suitable

specific activity

≥100 DEA units/mg protein

specific activity

-

specific activity

≥4000 units/mg protein

specific activity

30-60 units/mg protein (in glycine buffer)

form

lyophilized powder

form

buffered aqueous glycerol solution

form

solid

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ≥50%

composition

-

composition

-

composition

Protein, ~50% biuret


Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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