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Platelet-Derived Endothelial Cell Growth Factor human

Name: Platelet-Derived Endothelial Cell Growth Factor human
Brand: SIGMA
Price: 424 USD
Availability: InStock

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

PD-ECGF

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Size/SKU	Availability	Price
10 μg	Available to ship TODAYfromMILWAUKEE	$424.00

About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD01324253

Biological source:

human

Recombinant:

expressed in baculovirus infected Sf21 cells

Assay:

≥97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

49 kDa

Impurities:

<1 EU/mg

$424.00

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Properties

Product Name

Platelet-Derived Endothelial Cell Growth Factor human, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

5-30 ng/mL EC₅₀

mol wt

49 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/mg

UniProt accession no.

P01127

storage temp.

−20°C

Gene Information

human ... PDGFA(100126841), TYMP(1890)

Description

General description

Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) is an endothelial cell mitogen which was originally purified from human platelets. In contrast with the endothelial mitogens of the fibroblast growth factor (FGF) family, PD-ECGF is an endothelial cell growth factor initially purified from human platelets, which does not bind to heparin and does not stimulate the proliferation of fibroblasts. This is in contrast with the effect of PDGF, which stimulates the growth of human foreskin fibroblasts, but is inactive on endothelial cells. PD-ECGF will stimulate endothelial cells in vitro and in vivo. PD-ECGF is chemotactic for bovine aortic endothelial cells. PD-ECGF does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice. PD-ECGF has a pI of 4.0-4.8 and a molecular weight of 49 kDa.

The biological activity of recombinant, human PD-ECGF is measured in a cell proliferation assay using human umbilical vein endothelial cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay

Application

PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice.

Biochem/physiol Actions

Platelet-derived endothelial cell growth factor is a 45 kDa endothelial cell mitogen of pI 4.0-4.8 originally purified from human platelets. PD-ECGF is chemotactic for bovine aortic endothelial cells, however it does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioalantoic membrane and vascularization of tumor cells in nude mice. PD-ECGF is highly expressed in some tumors.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

The proliferative activity of human PD-ECGF is measured in culture by using human umbilical vein endothelial cells.

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This Item	P3201	P4306	P3326
Sigma-Aldrich P5208 Platelet-Derived Endothelial Cell Growth Factor human	Sigma-Aldrich P3201 Platelet-Derived Growth Factor-BB human	Sigma-Aldrich P4306 Platelet-Derived Growth Factor-BB human	Sigma-Aldrich P3326 Platelet-Derived Growth Factor-AB human
description recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture	description PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
recombinant expressed in baculovirus infected Sf21 cells	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
biological source human	biological source human	biological source human	biological source human
assay ≥97% (SDS-PAGE)	assay ≥98% (HPLC and SDS-PAGE)	assay ≥97% (SDS-PAGE)	assay ≥97% (SDS-PAGE and N-terminal analysis)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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