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PLD35

GenElute™ Plasmid Midiprep Kit

greener alternative

sufficient for 35 purifications

Sinónimos:

Gen Elute, GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit

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Tamaño de envaseSKUDisponibilidadPrecio
1 kit

Disponible para enviar HOYdesdeMILWAUKEE

$396.00

Acerca de este artículo

NACRES:
NA.52
UNSPSC Code:
41105501

$396.00

Disponible para enviar HOYDetalles

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Servicio técnico
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usage

sufficient for 35 purifications

Quality Level

200

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

test parameters

: ~45 min kit run time, sample volume: 5-40 mL bacterial culture

greener alternative category

Aligned,

storage temp.

15-25°C

General description

Click here to view the kit protocol
The GenElute Plasmid Midiprep Kit offers a simple, rapid, cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica- binding technology and the convenience of a spin column format, up to 300 μg of plasmid DNA can be recovered from 20-40 ml of Luria Broth (LB) in about 45 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in such downstream applications as restriction enzyme digestion, cloning, PCR, transcription and automated sequencing.
The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination detected by agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, and transfection.

Biochem/physiol Actions

Bacterial cells are harvested via centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorbtion of DNA onto silca in the presence of high salts. Contaminants are then removed by a simple wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer. The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination.

Other Notes

GenElute Plasmid Midi- and Maxiprep Kits offer a simple, rapid and cost-effective method for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology with the convenience of a spin column format and deliver up to 350 μg (Midi) and 1.2 mg (Maxi) yield of high-copy plasmid DNA.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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usage

sufficient for 35 purifications

usage

sufficient for 25 purifications

usage

sufficient for 35 purifications

usage

sufficient for 70 purifications

technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

greener alternative category

Aligned,

greener alternative category

, Aligned

greener alternative category

Aligned,

greener alternative category

, Aligned

test parameters

: ~45 min kit run time, sample volume: 5-40 mL bacterial culture

test parameters

sample volume: 1-5 mL bacterial culture

test parameters

: <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture

test parameters

: <30 min kit run time, sample volume: 1-5 mL

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios
  • R1149
    Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )
    SDS
  • C2112
    Column Preparation Solution
    SDS
  • R6148
    RNase A solution
    SDS
  • L1912
    Lysis Solution
    SDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Clase de almacenamiento

3 - Flammable liquids

wgk

WGK 3

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Certificados de análisis (COA)

Lot/Batch Number
0000331156
0000331156
SLCQ2182
SLCL7193
SLCK9355

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SKUGTIN
PLD35-1KT04061836680978

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