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R0278

RIPA Buffer

Synonym(s):

Radioimmunoprecipitation assay buffer

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50 mL

Estimated to ship TODAYfromMILWAUKEE

$112.00
500 mL

Estimated to ship TODAYfromMILWAUKEE

$574.00

About This Item

UNSPSC Code:
12161703
NACRES:
NA.56
MDL number:
MFCD02100484

$112.00

Estimated to ship TODAYDetails

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description

protease free

Quality Segment

form

liquid

packaging

pkg of 50 mL, pkg of 500 mL

technique(s)

immunoprecipitation (IP): suitable

color

colorless

input

mammalian sample(s)

pH

7.8-8.2 (25 °C, 1 ×)

storage temp.

2-8°C

General description

RIPA (Radio-Immunoprecipitation Assay) Buffer is supplied as a ready to use solution that requires no preparation. It minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. It is widely used in applications such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this buffer.
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

Application

RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview™ Affinity Gels.
RIPA Buffer has been used as a reagent for cell lysis in the following experimental studies:
  • Fibroblast-based deep phenotyping of the peripheral tissue, which facilitates the mechanistic disease stratification in sporadic Parkinson′s disease (sPD).
  • Demonstrating the inhibition of oxidative phosphorylation (OXPHOS), which can activate the mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis in human lung epithelial cells.

Features and Benefits

1 mL of the RIPA Buffer is used to lyse cells from one 100 mm culture dish (0.5 to 5 * 107 cells) of most adherent mammalian cell lines.
Minimizes non-specific protein binding interactions to ensure low background.

Other Notes

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.

Legal Information

IGEPAL is a registered trademark of Syensqo
EZview is a trademark of Sigma-Aldrich Co. LLC

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This Item
P361968268T0449
RIPA Buffer

Sigma-Aldrich

R0278

RIPA Buffer

Phosphate Buffer Solution 1.0 M, pH 7.4 (25 °C)

Sigma-Aldrich

P3619

Phosphate Buffer Solution

Imidazole buffer Solution BioUltra, 1 M in H2O

Sigma-Aldrich

68268

Imidazole buffer Solution

Triethanolamine Buffer solution

Sigma-Aldrich

T0449

Triethanolamine Buffer solution

description

-

description

1.0 M, pH 7.4 (25 °C)

description

BioUltra, 1 M in H2O

description

-

technique(s)

immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

pH

7.8-8.2 (25 °C, 1 ×)

pH

7.4 (25 °C)

pH

9.5±0.5

pH

-

color

colorless

color

-

color

-

color

-

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Pictograms

Exclamation mark
GHS07

Storage Class

12 - Non Combustible Liquids

Signal Word

Danger

Hazard Codes

H319,H412,EUH430

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2

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Questions

1–10 of 12 Questions  

  1. we bought a bottle of RIPA buffer and want to make alliquots of this bottle. Can we store the alliquots of the buffer by -20? Or is it better to keep them between 2°C-8°C ?

    1 answer

    1. It is recommended to store the product at 2–8C. Studies have not been performed on aliquots and to determine if refrigerated storage will extend the shelf life of this product. We would recommend searching the general research literature or contacting researchers who routinely use this product to see if solution stability information is available. If a recommended storage is not given in the literature, it is usually best to make solutions fresh for use. If you decide to store the aliquots at -20°C, we would recommend validating activity of the supplemented buffer after freezing and avoid any thawing/freezing cycle. Please refer to this technical bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  2. Is this RIPA lysis buffer capable of extracting nuclear protein in mammalian cells?

    1 answer

    1. Yes, RIPA buffer typically lyses cell nuclei. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  3. Can I use R0278 for human protein extract for lymphocytes?

    1 answer

    1. This product has not been tested for protein extract from human lymphocytes. References using RIPA buffer with human lymphocytes can be found here:
      https://www.sciencedirect.com/science/article/pii/S0021925818639675
      https://www.sciencedirect.com/science/article/pii/S000887491530037X?via%3Dihub

      Helpful?

  4. Can I use the Ripa Buffer to extract yeast protein and make the Bradford assay on it?

    1 answer

    1. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  5. How long can the RIPA buffer be stored in 4C? I stored it for almost 6 years, can I still use it?

    1 answer

    1. Regarding stability in the recommended temperature of 2-8 °C, this product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  6. Can this be used for Western blot experiments?

    1 answer

    1. Yes, this product would be suitable for western blotting.

      Helpful?

  7. Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?

    1 answer

    1. This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.

      Helpful?

  8. After preparing a lysate using Product R0278, RIPA Buffer, how should it be stored? And for how long can the lysate be stored?

    1 answer

    1. Lysates containing the appropriate inhibitor(s) can be stored at -70°C for up to a year.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?

    1 answer

    1. R0278 can be used on tissue, if it is well homogenized.

      Helpful?

1–10 of 12 Questions  

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