The composition of lysis buffer R0278 is 150 mM sodium chloride, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, and 50 mM Tris at pH 8.0. The buffer does not contain protease or phosphatase inhibitors. Protease or phosphatase inhibitors may be added to protect against possible proteolysis.
R0278
RIPA Buffer
Synonym(s):
Radioimmunoprecipitation assay buffer
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| Size/SKU | Availability | Price |
|---|---|---|
50 mL | Estimated to ship TODAYfromMILWAUKEE | $112.00 |
500 mL | Estimated to ship TODAYfromMILWAUKEE | $595.00 |
About This Item
$112.00
Estimated to ship TODAYDetails
description
protease free
Quality Segment
form
liquid
packaging
pkg of 50 mL, pkg of 500 mL
technique(s)
immunoprecipitation (IP): suitable
color
colorless
input
mammalian sample(s)
pH
7.8-8.2 (25 °C, 1 ×)
storage temp.
2-8°C
General description
RIPA (Radio-Immunoprecipitation Assay) Buffer is supplied as a ready to use solution that requires no preparation. It minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. It is widely used in applications such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this buffer.
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
Application
RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview™ Affinity Gels.
RIPA Buffer has been used as a reagent for cell lysis in the following experimental studies:
- Fibroblast-based deep phenotyping of the peripheral tissue, which facilitates the mechanistic disease stratification in sporadic Parkinson′s disease (sPD).
- Demonstrating the inhibition of oxidative phosphorylation (OXPHOS), which can activate the mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis in human lung epithelial cells.
Features and Benefits
1 mL of the RIPA Buffer is used to lyse cells from one 100 mm culture dish (0.5 to 5 * 107 cells) of most adherent mammalian cell lines.
Minimizes non-specific protein binding interactions to ensure low background.
Other Notes
Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.
Legal Information
IGEPAL is a registered trademark of Syensqo
EZview is a trademark of Sigma-Aldrich Co. LLC
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This Item | |
|---|---|
| description - | description OmniPur® Grade, 100X Liquid Concentrate, pH 8.0 |
| technique(s) immunoprecipitation (IP): suitable | technique(s) - |
| Quality Level 200 | Quality Level 100 |
| form liquid | form liquid |
| storage temp. 2-8°C | storage temp. 15-25°C |
| pH 7.8-8.2 (25 °C, 1 ×) | pH - |
| color colorless | color haze-free colorless |
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Storage Class
12 - Non Combustible Liquids
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
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Could you please provide the composition of your RIPA lysis buffer, including its main components and whether it contains protease or phosphatase inhibitors?
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we bought a bottle of RIPA buffer and want to make alliquots of this bottle. Can we store the alliquots of the buffer by -20? Or is it better to keep them between 2°C-8°C ?
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It is recommended to store the product at 2–8C. Studies have not been performed on aliquots and to determine if refrigerated storage will extend the shelf life of this product. We would recommend searching the general research literature or contacting researchers who routinely use this product to see if solution stability information is available. If a recommended storage is not given in the literature, it is usually best to make solutions fresh for use. If you decide to store the aliquots at -20°C, we would recommend validating activity of the supplemented buffer after freezing and avoid any thawing/freezing cycle. Please refer to this technical bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf
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Is this RIPA lysis buffer capable of extracting nuclear protein in mammalian cells?
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Yes, RIPA buffer typically lyses cell nuclei. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf
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Can I use R0278 for human protein extract for lymphocytes?
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This product has not been tested for protein extract from human lymphocytes. References using RIPA buffer with human lymphocytes can be found here:
https://www.sciencedirect.com/science/article/pii/S0021925818639675
https://www.sciencedirect.com/science/article/pii/S000887491530037X?via%3DihubHelpful?
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Can I use the Ripa Buffer to extract yeast protein and make the Bradford assay on it?
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RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf
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How long can the RIPA buffer be stored in 4C? I stored it for almost 6 years, can I still use it?
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Regarding stability in the recommended temperature of 2-8 °C, this product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Can this be used for Western blot experiments?
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Yes, this product would be suitable for western blotting.
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Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?
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This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.
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How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?
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RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins. The exact formulation of C2978, however, is proprietary.
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After preparing a lysate using Product R0278, RIPA Buffer, how should it be stored? And for how long can the lysate be stored?
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Lysates containing the appropriate inhibitor(s) can be stored at -70°C for up to a year.
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