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Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal

Name: Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal
Brand: SIGMA
Price: 571 USD
Availability: InStock

clone RabT-50, purified from hybridoma cell culture

Synonym(s):

Mouse Anti-Rabbit IgG

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$571.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

RabT-50, monoclonal

Application:

ELISA (i), ICC, WB

Citations:

$571.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RabT-50, monoclonal

form

buffered aqueous solution

species reactivity

rabbit

should not react with

human, horse, bovine, cat, rat, chicken, pig, goat, monkey

concentration

2.5-5.0 mg/mL (antibody)

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody, western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).

Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.

Immunogen

Enriched fraction of rabbit IgG light chain.

Application

Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:

immunoblotting
immunoprecipitation
western blotting

Biochem/physiol Actions

Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R2655	A3673	A4541
Sigma-Aldrich R3155 Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich R2655 Anti-Rabbit IgG, Native antibody, Mouse monoclonal	Sigma-Aldrich A3673 Anti-Mouse IgG (γ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A4541 Anti-Biotin−Peroxidase antibody produced in goat
description clone RabT-50, purified from hybridoma cell culture	description clone RabT-50, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
conjugate peroxidase conjugate	conjugate unconjugated	conjugate peroxidase conjugate	conjugate peroxidase conjugate
species reactivity rabbit	species reactivity rabbit	species reactivity mouse	species reactivity -
technique(s) western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody., indirect ELISA: suitable, immunocytochemistry: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody	technique(s) indirect ELISA: suitable, western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane, immunocytochemistry: suitable	technique(s) direct ELISA: 1:15,000	technique(s) direct ELISA: 1:2,000
biological source mouse	biological source mouse	biological source goat	biological source goat
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody
isotype IgG1	isotype IgG1	isotype -	isotype -

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

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