Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

R4526

Reference Dye for Quantitative PCR

100 ×, solution

Synonym(s):

Reference dye for qPCR, ROX

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
0.3 mL

Available to ship TODAYfromMILWAUKEE

$19.90
5 mL

Available to ship TODAYfromMILWAUKEE

$300.00

About This Item

NACRES:
NA.55
UNSPSC Code:
41106300

$19.90

Available to ship TODAYDetails

​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

solution

Quality Segment

300

usage

sufficient for ≥600 reactions

feature

PCR Additives

concentration

100 ×

technique(s)

PCR: suitable, whole genome amplification: suitable

color

red

input

crude DNA

solubility

water: soluble

storage temp.

2-8°C

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
230914SCT036539141
Reference Dye for Quantitative PCR 100 ×, solution

Sigma-Aldrich

R4526

Reference Dye for Quantitative PCR

Chymotrypsin Substrate II, Fluorogenic Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

Sigma-Aldrich

230914

Chymotrypsin Substrate II, Fluorogenic

BioTracker 625 Red GSH Dye Live cell imaging dye for glutathione (GSH) that detects reactive oxygen species (ROS) free radicals.

Sigma-Aldrich

SCT036

BioTracker 625 Red GSH Dye

Proteasome Substrate II, Fluorogenic Fluorogenic proteasome substrate.

Sigma-Aldrich

539141

Proteasome Substrate II, Fluorogenic

description

100 ×, solution

description

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

description

Live cell imaging dye for glutathione (GSH) that detects reactive oxygen species (ROS) free radicals.

description

Fluorogenic proteasome substrate.

technique(s)

PCR: suitable, whole genome amplification: suitable

technique(s)

-

technique(s)

cell based assay: suitable

technique(s)

-

concentration

100 ×

concentration

-

concentration

-

concentration

-

color

red

color

white

color

-

color

white

solubility

water: soluble

solubility

acetonitrile: water (1:1): soluble, DMSO: soluble

solubility

-

solubility

DMSO: 50 mg/mL

form

solution

form

lyophilized solid

form

-

form

solid

Quality Level

300

Quality Level

100

Quality Level

-

Quality Level

100

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

ppe

Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters