Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.
Application
Reference Dye for Quantitative PCR has been used:
in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
for analyzing the degree of cellular DNA contamination by qPCR
Other Notes
Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.
