Yes, SYPRO Ruby Protein Blot Stain works with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes. For more information, please access the Product Data Sheet which can be found in the DOCUMENTATION section under the 'More Documents' tab:
https://www.sigmaaldrich.com/product/sigma/s4942#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/369/103/s4817dat.pdf
S4942
SYPRO™ Ruby Protein Gel Stain
Synonym(s):
protein gel stain, SYPRO™ dye
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| Size/SKU | Availability | Price |
|---|---|---|
200 mL | Available to ship TODAY | $198.00 |
1 L | Available to ship TODAY | $675.00 |
5 L | Available to ship TODAY | $2,740.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41105322
$198.00
Available to ship TODAYDetails
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Ships Every 4 weeks
shelf life
≥6 mo. (when stored at room temperature and protected from light)
Quality Segment
technique(s)
protein electrophoresis: suitable, protein staining: suitable
fluorescence
λex 280,450 nm; λem 610 nm
General description
SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
- uses a simple staining protocol, with no possibility of overstaining
- delivers a linear quantitation range of over three orders of magnitude
- shows less protein-to-protein variability
- stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
- will not stain extraneous nucleic acids
- does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
Application
Legal Information
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
Disclaimer
Protect from light.
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description 10,000 × in DMSO |
| technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) - |
| fluorescence λex 280,450 nm; λem 610 nm | fluorescence λex 300,470 nm; λem 570 nm | fluorescence - | fluorescence - |
| shelf life ≥6 mo. (when stored at room temperature and protected from light) | shelf life ≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.) | shelf life - | shelf life - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Salve Sybro ruby è utilizzabile per colorare membrane blot PVDF?
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