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S5512

Streptavidin−Peroxidase from Streptomyces avidinii

lyophilized powder

Synonym(s):

Streptavidin−HRP from Streptomyces avidinii

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0.1 mg

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$77.40
0.5 mg

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$224.00
1 mg

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$380.00
2 mg
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$700.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00132387

$77.40

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conjugate

peroxidase conjugate

Quality Segment

200

form

lyophilized powder

composition

Protein, ≥70% E1%/280

technique(s)

direct ELISA: 1:50,000

storage temp.

−20°C

General description

Streptavidin derives its name from its bacterial source Streptomyces avidinii and from the hen egg-white protein, avidin, which has high affinity to biotin. Its homologous core shares 33% sequence similarity with avidin, as well as sharing a common tetrameric structure. It is a crystalline tetrameric protein, with a molecular weight of 4*15000Da. It binds four molecules of biotin. Streptavidin lacks carbohydrate and sulfur-containing amino acids.
Steptavidin is coupled to horseradish peroxidase (HRP) using citrate buffer, pH 6.0, and a modified published procedure to form a 1:1 conjugate. HRP has a molecular mass of ~44kDa and steptavidin has a molecular mass of ~60kDa.
Streptavidin is considered as a high-affinity biotin-binding agent, which shows resistance to extreme pH, detergents temperature, denaturants and enzymes, hence it is used in molecular biology and bionanotechnology.

Application

Streptavidin- Peroxidase from Streptomyces avidinii has been used for ELISA (enzyme linked immunosorbent assay) and Enzyme-Linked ImmunoSpot (ELISPOT).
Streptavidin-Peroxidase from Streptomyces avidinii has used as a secondary reagent for detection of biotinylated antibodies in standard ELISA, immunoblotting, and immunocytochemistry procedures.

Biochem/physiol Actions

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization, and affinity chromatography.

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing citrate buffer salts.

Preparation Note

Labeled with Type VI peroxidase by a modification of the method of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
Purified by affinity chromatography.

Analysis Note

The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/mL stock in buffer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Streptavidin−Peroxidase from Streptomyces avidinii lyophilized powder

Sigma-Aldrich

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Streptavidin−Peroxidase from Streptomyces avidinii

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii lyophilized powder, ≥700 DEA units/mg protein

Sigma-Aldrich

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Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

Streptavidin from Streptomyces avidinii recombinant, expressed in E. coli, lyophilized powder

Sigma-Aldrich

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Streptavidin from Streptomyces avidinii

Streptavidin from Streptomyces avidinii essentially salt-free, lyophilized powder, ≥13 units/mg protein

Sigma-Aldrich

S4762

Streptavidin from Streptomyces avidinii

description

lyophilized powder

description

lyophilized powder, ≥700 DEA units/mg protein

description

recombinant, expressed in E. coli, lyophilized powder

description

essentially salt-free, lyophilized powder, ≥13 units/mg protein

technique(s)

direct ELISA: 1:50,000

technique(s)

-

technique(s)

-

technique(s)

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ≥70% E1%/280

composition

Protein, ≥40% biuret

composition

-

composition

-

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

-

conjugate

-

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