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Anti-Human IgE antibody, Mouse monoclonal

Name: Anti-Human IgE antibody, Mouse monoclonal
Brand: SIGMA
Price: 552 USD
Availability: InStock

clone GE-1, purified from hybridoma cell culture

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Synonym(s):

Anti-Immunoglubulin E

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$552.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Clone:

GE-1, monoclonal

Species reactivity:

human

Application:

ELISA, IP

Citations:

$552.00

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Properties

biological source

mouse

Quality Segment

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GE-1, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating, immunoblotting: suitable, immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.

Immunogen

purified human IgE myeloma protein

Application

Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.

Biochem/physiol Actions

Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Show Differences

1 of 1

This Item	I6510	401152	SAB4200759
Sigma-Aldrich SAB4200741 Anti-Human IgE antibody, Mouse monoclonal	Sigma-Aldrich I6510 Monoclonal Anti-Human IgE antibody produced in mouse	Sigma-Aldrich 401152 Human IgE, Myeloma	Sigma-Aldrich SAB4200759 Anti-Human IgG3 antibody, Mouse monoclonal
description clone GE-1, purified from hybridoma cell culture	description clone GE-1, ascites fluid	description This Human IgE, Myeloma is validated for use as an Immunoassay standard.	description clone HP-6050, purified from hybridoma cell culture
species reactivity human	species reactivity -	species reactivity -	species reactivity human
biological source mouse	biological source mouse	biological source (Human myeloma)	biological source mouse
antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form -	antibody form purified from hybridoma cell culture
clone GE-1, monoclonal	clone GE-1, monoclonal	clone -	clone HP-6050, monoclonal
technique(s) immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma, ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating, immunoblotting: suitable	technique(s) indirect ELISA: 1:5,000	technique(s) -	technique(s) immunoprecipitation (IP): 1-2.5 μg/test using Human IgG3 myeloma, indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml Human IgG3 myeloma for coating
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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