Key Documents

View All Documentation

Carboxypeptidase A

Name: Carboxypeptidase A
Brand: SIGMA
Price: 53.6 USD
Availability: InStock

Cleaves C-terminal amino acids, suitable for Mass Spectrometry, from bovine pancreas

Synonym(s):

Carboxypeptidase A, Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

Select a Size

Change View

Size/SKU	Availability	Price
500 units	Check Cart for Availability	$53.60
2500 units	Check Cart for Availability	$154.00
5000 units	Check Cart for Availability	$259.00

About This Item

CAS Number:

11075-17-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.17.1 (BRENDA, IUBMB)

MDL number:

MFCD00130718

Specific activity:

≥20 units/mg protein

Biological source:

bovine pancreas

$53.60

Check Cart for Availability

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Carboxypeptidase A from bovine pancreas, (Type II-PMSF treated), ≥20 units/mg protein

biological source

bovine pancreas

Quality Segment

200

form

aqueous suspension

quality

(Type II-PMSF treated)

specific activity

≥20 units/mg protein

mol wt

~35 kDa

purified by

2× crystallization

technique(s)

protein mass spectrometry: suitable

impurities

≤0.05 BTEE units/mg protein chymotrpsin, ≤10 BAEE units/mg protein trypsin

storage temp.

2-8°C

Description

General description

Carboxypeptidase A (CPA) is a secreted protease is liberated after the activation of mast cells to facilitate acute anaphylaxis. Carboxypeptidase A has a long half-life in vivo, when compared to other secreted proteases.

Application

Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.

Carboxypeptidase A from bovine pancreas has been used in in vitro simulated digestion.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.†

Preparation Note

Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.

Analysis Note

Protein determined by E1%/278

Other Notes

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	C9268	C1261	C5233
Sigma-Aldrich SAE0046 Carboxypeptidase A from bovine pancreas	Sigma-Aldrich C9268 Carboxypeptidase A from bovine pancreas	Sigma-Aldrich C1261 Carboxypeptidase A−Agarose	Sigma-Aldrich C5233 Carboxypeptidase B from human pancreas
description (Type II-PMSF treated), ≥20 units/mg protein	description (Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution	description ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas	description 50-55 units/mg protein carboxypeptidase B
specific activity ≥20 units/mg protein	specific activity ≥50 units/mg protein	specific activity ≥6 units/mL packed gel, 25 °C	specific activity 50-55 units/mg protein carboxypeptidase B
biological source bovine pancreas	biological source -	biological source enzyme from bovine pancreas	biological source human pancreas
form aqueous suspension	form ready-to-use solution	form ammonium sulfate suspension	form solution
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
mol wt ~35 kDa	mol wt ~35 kDa	mol wt ~35,250	mol wt -
purified by 2× crystallization	purified by 2× crystallization	purified by -	purified by -

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.