The Certificates of Analysis confirm that the Lyticase product from Arthrobacter luteus has an activity of ≥2000 units/mg protein. Batch-specific information on units/mg protein is available for reference, but the total mass of Lyticase provided in the vial is not a specification tested for batch release. Information regarding the preparation of stock solutions and storage/stability recommendations can be found in the Product Data Sheet.
SAE0098
Lyticase from Arthrobacter luteus
free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder
Synonym(s):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
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| Size/SKU | Availability | Price |
|---|---|---|
20 kU | Available to ship TODAYfromMILWAUKEE | $171.00 |
About This Item
Specific activity:
≥2000 units/mg protein
Biological source:
bacterial (Arthrobacter luteus)
biological source
bacterial (Arthrobacter luteus)
Quality Segment
form
lyophilized powder
specific activity
≥2000 units/mg protein
feature
DNA free
technique(s)
cell based assay: suitable
input
plant, fungi and insect sample(s)
plant, fungi and insect null
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
General description
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Other Notes
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| description free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder | description lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret | description Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein | description lyophilized powder, ≥200 units/mg solid |
| biological source bacterial (Arthrobacter luteus) | biological source bacterial (Arthrobacter luteus) | biological source bacterial (Arthrobacter luteus) | biological source bacterial (Arthrobacter luteus) |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - | technique(s) cell based assay: suitable |
| specific activity ≥2000 units/mg protein | specific activity ≥2,000 units/mg protein | specific activity ≥2000 units/mg protein | specific activity ≥200 units/mg solid |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| suitability suitable for cell lysis | suitability suitable for cell lysis | suitability - | suitability suitable for cell lysis |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the total amount of Lyticase in this product? The only information is 20000 units per mg. Should we assume that the content is 1mg? There cleary a huge lack of information on this product regarding quantity, resuspension, stability...
1 answer-
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What recommendations are available for the reconstitution of Lyticase? Should it be prepared in water or a buffer? Can you provide general information as well as specific information for product SAE0098?
1 answer-
Solutions of lyticase can be prepared in DNA-free water. One publication reports the preparation of stock solutions of lyticase at 5 mg/mL in 1 M sorbitol with 0.1 M EDTA, pH 8.0, with storage of these stock solutions at –20 C in frozen aliquots. However, this method has not been tested by us. The lyticase may also be dissolved in 50 mM potassium phosphate buffer, pH 7.5, with 50% glycerol and stored at -20 deg. Solutions of Lyticase are not considered to be stable, and it is recommended that solutions be prepared fresh and not stored as stock solutions. We do not have information on the long-term stability if stored at -20 C. We would advise using it within a period of 1 month if stored at -20 C in aliquots.
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