Transglutaminases (TG) are a family of enzymes that catalyze isopeptide bond formation. This bond formation occurs between the γ-carboxyamide group of glutamine and various primary amines (primarily the ε-amino group of lysine). The resulting intermolecular or intramolecular cross-linking of transglutaminase is highly stable and shows high resistance to proteolytic degradation. The transglutaminase crosslinking activity support the formation of blood clots , skin and hair. On the other hand, TG is now being implicated in Celiac disease as well as in Huntington and Parkinson diseases. Historically, Microbial transglutaminase has been heavily used in the food industry. Microbial TG is often preferred for newer applications (such as site-specific protein modification and antibody drug conjugation) due to its small molecular weight and lack of calcium dependency when compared to the mammalian forms.
Application
Microbial Transglutaminase has been used for:
Protein cross-linking & site-specific labeling
Antibody Drug Conjugation
3D bioprinting bioink preparation
Food related immunogenicity/pathogenicity related research
Features and Benefits
Small (~38kDa) and calcium independent enzyme
Highly purified lyophilized enzyme
Consistent and reproducible activity
Characterized for endotoxin content
Preparation Note
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices
Store the freeze-dried product at –20 °C. It is recommended to store the reconstituted protein in working aliquots at –20 °C to avoid repeated freeze/ thaw cycles.
Hi, I have purchased Microbial Transglutaminase lyophilized powder, >12 units per mg (SAE0159-25UN). How can I prepare its stock? How much volume of solvent (for example PBS) should I add in the vial containing enzyme powder?
1 answer
Technical Support
·2 years ago
Reconstitute this product in 20 mM Tris-HCl containing 150 mM NaCl, in Molecular Biology grade water, pH 8 at 0.14 units per microliter. The unit per milligram activity is lot-specific and available in the product Certificate of Analysis. Further usage instructions are available in the product datasheet at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/339/389/sae0159pis-ms.pdf
Bonjour,
Est-il possible de savoir la masse totale d'enzyme dans le produit ? Les 25 units correspondent bien à l'activité de la poudre (mélange enzyme + excipients) ?
1 answer
Technical Support
·2 years ago
The total mass of product in each vial varies from lot to lot due to variability in the protein content and the activity per milligram protein. The vial contents may be calculated based on information reported in the Certificate of Analysis. A review of recent lots reveals a range of 14 - 30% protein with an activity range of 14 -38 units per milligram protein.
