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SRP3239

GDNF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

ATF-1, Glial-Derived Neurotrophic Factor

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10 μg
Usually ships in 2 weeks.
$386.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$386.00


Usually ships in 2 weeks.

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biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

mol wt

15 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... GDNF(25453)

General description

GDNF (glial cell derived neurotrophic factor) is a member of the transforming growth factor-β (TGF) superfamily. It is a potent neurotrophic factor which functions as a ligand for the multicomponent receptor complex containing GFRα1 (GDNF family receptor α1) and Ret receptor tyrosine kinase or neuronal cell adhesion molecule (NCAM). Rat GDNF is a homodimer linked by a disulfide bridge, and contains two glycosylated polypeptide chains that are released from the 211-residue-long precursor following a proteolytic cleavage.
The functional rat GDNF ligand is a disulfide-linked homodimer, of two 15.0kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Biochem/physiol Actions

GDNF (glial cell derived neurotrophic factor) enhances motoneuron survival, and facilitates the survival of midbrain dopaminergic neurons which are damaged in degenerative diseases, such as Parkinson′s. In vivo and in vitro, the interaction of this protein with GDNF family receptor α1 and its co-receptor Ret tyrosine kinase, results in elevated neurite extension and survival of motor and sensory neurons. In rats with neuropathic pain, the injection of this protein in the locus coeruleus (LC) produces prolonged analgesia by increasing descending noradrenergic inhibition.

Physical form

Lyophilized from 10 mM Sodium Citrate.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI

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G1401SRP3309SRP3014
description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥97% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

rat

biological source

rat

biological source

human

biological source

human

form

lyophilized

form

lyophilized powder

form

lyophilized

form

lyophilized

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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