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T1270

Thiamine hydrochloride

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Synonym(s):

Aneurine hydrochloride, Vitamin B1 hydrochloride

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Size/SKUAvailabilityPrice
25 g

Available to ship TODAYfromMILWAUKEE

$77.30
100 g

Available to ship TODAYfromMILWAUKEE

$159.00

About This Item

Empirical Formula (Hill Notation):
C12H17ClN4OS · HCl
CAS Number:
67-03-8
Molecular Weight:
337.27
NACRES:
NA.75
PubChem Substance ID:
24899952
eCl@ss:
34058006
UNSPSC Code:
12352207
EC Number:
200-641-8
MDL number:
MFCD00012780
Beilstein/REAXYS Number:
3851771

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biological source

synthetic (organic)

Quality Segment

200

product line

BioReagent

assay

≥99.0% (HPLC)

form

powder

mol wt

Mw 337.27 g/mol

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

color

white

mp

250 °C (dec.) (lit.)

solubility

H2O: 50 mg/mL, clear, colorless

application(s)

agriculture

SMILES string

CC1=NC(N)=C(C[N+]2=CSC(CCO)=C2C)C=N1.Cl.[Cl-]

InChI

1S/C12H17N4OS.2ClH/c1-8-11(3-4-17)18-7-16(8)6-10-5-14-9(2)15-12(10)13;;/h5,7,17H,3-4,6H2,1-2H3,(H2,13,14,15);2*1H/q+1;;/p-1

InChI key

DPJRMOMPQZCRJU-UHFFFAOYSA-M

General description

Listen to an Audio Overview
Thiamine or Vitamin B1 is a positively charged and essential constituent for all tissues. It is present at higher levels in skeletal muscles, liver, kidney, heart, and brain. Thiamine is composed of a pyrimidine ring linked to a thiazole ring via a methylene bridge.

Application

Thiamine hydrochloride has been used as a component:
  • of the vitamin-concentrated stock solution for the preparation of Hv-Ca medium for culturing Haloferax volcanii
  • of the tris-acetate-phosphate (TAP) medium for culturing Chlamydomonas nivalis
  • of modified Bold 3N medium for culturing Chlorella minutissima

Biochem/physiol Actions

Thiamine is converted to thiamine diphosphate post-movement into the cellular membranes by thiamine diphosphokinase. Thiamine diphosphate, an active cofactor is essential for the mechanism of many enzymes involved in the citric acid cycle, the glycolytic pathway, the degradation of branched-chain amino acids, and the pentose phosphate pathway. Thiamine is involved in converting food into energy required for functioning the central and peripheral nervous systems. Lower levels of this vitamin are associated with delirium, Wernicke′s encephalopathy, and Wernicke-Korsakoff syndrome. Thiamine hydrochloride is required to support energy metabolism and amino acid synthesis in cultured cells. It is present in many classical and serum-free formulations.

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This Item
Y0000467W33220804573
Thiamine hydrochloride BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Sigma-Aldrich

T1270

Thiamine hydrochloride

Thiamine hydrochloride European Pharmacopoeia (EP) Reference Standard

Y0000467

Thiamine hydrochloride

Thiamine hydrochloride ≥98%, FCC, FG

Sigma-Aldrich

W332208

Thiamine hydrochloride

Thiamine hydrochloride tested according to Ph. Eur.

Sigma-Aldrich

04573

Thiamine hydrochloride

description

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

description

European Pharmacopoeia (EP) Reference Standard

description

≥98%, FCC, FG

description

tested according to Ph. Eur.

technique(s)

cell culture | plant: suitable, cell culture | mammalian: suitable, cell culture | insect: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

≥99.0% (HPLC)

assay

-

assay

≥98%

assay

98.5-101.0% anhydrous basis

biological source

synthetic (organic)

biological source

-

biological source

synthetic

biological source

synthetic

form

powder

form

-

form

powder or crystals

form

solid

application(s)

agriculture

application(s)

pharmaceutical (small molecule)

application(s)

flavors and fragrances

application(s)

-

solubility

H2O: 50 mg/mL, clear, colorless

solubility

-

solubility

H2O: 1 g/10 mL, clear, colorless

solubility

diethyl ether: insoluble, propylene glycol: soluble, benzene: insoluble, glycerol: soluble 1 gm in 18 ml, alcohol: soluble 1 gm in 315 ml (Absolute), chloroform: insoluble, hexane: insoluble, methanol: very soluble, alcohol: soluble 1 gm in 100 ml (95%), water: soluble 1 g/mL

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

  1. Combien de temps est stable la thiamine une fois resuspendue dans de l'eau milliQ ?

    1 answer

    1. The stability of this product in solution has not been specifically investigated but is expected to be quite robust. This material is a component in a number of classical liquid culture media products, which are assigned a shelf-life of three years. Please see the link below to review a sampling of these media formulations:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/282/935/d9785for.pdf

      Please see the link below to review a publication that offers additional information on the stability of this material in solution:
      https://pubmed.ncbi.nlm.nih.gov/34384471/

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