Several modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.
Application
TNM-FH insect medium has been used to culture Sf9 insect cells.
With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture
description
With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture
description
supplemented Grace′s, with 4.1 mM L-glutamine, with 3.33 g/L lactalbumin hydrolysate (LAH), with 3.33 g/L ultrafiltered yeastolate, liquid, sterile-filtered
description
With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture
