T3913
Tris-Borate-EDTA buffer
5× concentrate, powder blend
Synonym(s):
TBE buffer
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
1 L | Check Cart for Availability | $27.40 $20.55 |
5 x 1 L | Check Cart for Availability | $92.70 $69.52 |
About This Item
Quality Segment
form
powder blend
technique(s)
electrophoresis: suitable
impurities
DNase, RNase and NICKase, none detected
pH
8.1-8.5 (25 °C, 5 ×)
solubility
water: 85.1 g/L, clear, colorless
suitability
suitable for gel electrophoresis (after dilution to working concentration)
application(s)
diagnostic assay manufacturing
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
Application
It may be employed for the following studies:
- As buffer system for the evaluation of molecular weights of glycoproteins of ovalbumin, avidin and fetuin by sodium dodecyl sulfate-pore gradient electrophoresis.
- For the hemoglobin electrophoresis in starch gel.
- Preparation of buffer concentration gradient gel for the rapid determination of DNA sequence.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
Preparation Note
1 of 1
This Item | |||
|---|---|---|---|
| description 5× concentrate, powder blend | description BioReagent, Molecular Biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis | description 10× concentrate, BioReagent, Molecular Biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis | description DNase and RNase, none detected, BioReagent, suitable for electrophoresis, 10× concentrate |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder blend | form powder blend | form powder blend | form powder blend |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility water: 85.1 g/L, clear, colorless | solubility water: 85.1 g/L, clear, colorless | solubility - | solubility water: 64.2 g/L, clear, colorless |
| pH 8.1-8.5 (25 °C, 5 ×) | pH 8.1-8.5 (5 ×) | pH 8.1-8.5 | pH 8.1-8.5 |
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signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P2 (EN 143) respirator cartridges
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