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Trypsin−Agarose

Name: Trypsin−Agarose
Brand: SIGMA

buffered aqueous suspension, from bovine pancreas trypsin

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About This Item

UNSPSC Code:

12352204

eCl@ss:

42020142

NACRES:

NA.54

MDL number:

MFCD00082094

Biological source:

bovine pancreas (trypsin)

Concentration:

≥15 units/mL (packed gel)

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Properties

biological source

bovine pancreas (trypsin)

Quality Segment

200

form

buffered aqueous suspension

concentration

≥15 units/mL (packed gel)

extent of labeling

≥15 units per mL (packed gel)

matrix

beaded agarose

storage temp.

2-8°C

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. [1] Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA. [2]

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

Preparation Note

TPCK-treated

Other Notes

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

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1 of 1

This Item	T8802	T6763	T1763
Sigma-Aldrich T4019 Trypsin−Agarose	Sigma-Aldrich T8802 Trypsin from bovine pancreas	Sigma-Aldrich T6763 Trypsin Acetylated from bovine pancreas	Sigma-Aldrich T1763 Trypsin Agarose
biological source bovine pancreas (trypsin)	biological source bovine pancreas	biological source bovine pancreas	biological source bovine pancreas (trypsin)
form buffered aqueous suspension	form essentially salt-free, lyophilized powder	form powder	form buffered aqueous suspension
concentration ≥15 units/mL (packed gel)	concentration -	concentration -	concentration ≥15 units/mL (packed gel)
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
matrix beaded agarose	matrix -	matrix -	matrix cross-linked beaded agarose
extent of labeling ≥15 units per mL (packed gel)	extent of labeling -	extent of labeling -	extent of labeling ≥15 units per mL packed gel

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

64-19-7

CAS No.

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