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T4444

3, 3′,5 ,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, aqueous solution

Synonym(s):

TMB

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Size/SKUAvailabilityPrice
100 mL
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$140.00
4 x 100 mL
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$421.00

About This Item

CAS Number:
54827-17-7
UNSPSC Code:
12352204
PubChem Substance ID:
24900209
NACRES:
NA.83
MDL number:
MFCD00007748

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Product Name

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution

Quality Segment

form

aqueous solution

technique(s)

protein detection: suitable

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

General description

This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:
  • to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectant
  • to measure the levels of α-hemolysin toxin
  • in imaging bound IgGs

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This Item
0974387748T5525
3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA ready to use solution

Sigma-Aldrich

T4444

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA

3,3′,5,5′-Tetramethylbenzidine standard for GC

Supelco

09743

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥98.0% (NT)

Sigma-Aldrich

87748

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet

Sigma-Aldrich

T5525

3,3′,5,5′-Tetramethylbenzidine

description

ready to use solution

description

standard for GC

description

≥98.0% (NT)

description

tablet, 1 mg substrate per tablet

technique(s)

protein detection: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

UV/Vis spectroscopy: suitable, protein detection: suitable

technique(s)

protein detection: suitable

Quality Level

200

Quality Level

100

Quality Level

300

Quality Level

200

form

aqueous solution

form

-

form

powder

form

tablet

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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