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T5525

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, tablet

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

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Size/SKUAvailabilityPrice
50 tabs

Available to ship onOctober 14, 2026fromMILWAUKEE

$255.00
100 tabs

Available to ship onOctober 14, 2026fromMILWAUKEE

$435.00

About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
24900293
UNSPSC Code:
12352204
MDL number:
MFCD00007748
Beilstein/REAXYS Number:
2808541

$255.00

Available to ship onOctober 14, 2026Details

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Product Name

3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet

Quality Segment

form

tablet

technique(s)

protein detection: suitable

mp

168-171 °C (lit.)

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.

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This Item
09743860336T2885
3,3′,5,5′-Tetramethylbenzidine tablet, 1 mg substrate per tablet

Sigma-Aldrich

T5525

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine standard for GC

Supelco

09743

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥99%

Sigma-Aldrich

860336

3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)

Sigma-Aldrich

T2885

3,3′,5,5′-Tetramethylbenzidine

description

tablet, 1 mg substrate per tablet

description

standard for GC

description

≥99%

description

≥98% (TLC)

technique(s)

protein detection: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

protein detection: suitable

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

form

tablet

form

-

form

powder

form

powder

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

mp

168-171 °C (lit.)

mp

166-171 °C, 168-171 °C (lit.)

mp

168-171 °C (lit.)

mp

168-171 °C (lit.)

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Codes

H302,H351,H413

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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