If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
T7024
N,N,N′,N′-Tetramethylethylenediamine
BioReagent, Molecular Biology, ≥99% (GC)
Synonym(s):
1,2-Bis(dimethylamino)ethane, TEMED, TMEDA
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
25 mL | Check Cart for Availability | $65.40 |
50 mL | Check Cart for Availability | $109.00 |
100 mL | Check Cart for Availability | $178.00 $151.30 |
About This Item
$65.40
grade
Molecular Biology
Quality Segment
vapor pressure
ca.19.19 hPa
product line
BioReagent
assay
≥99% (GC)
expl. lim.
9.08 %
technique(s)
electrophoresis: suitable, protein electrophoresis: suitable
refractive index
n20/D 1.4179 (lit.)
bp
120-122 °C (lit.)
mp
−55 °C (lit.)
density
0.775 g/mL at 20 °C (lit.)
foreign activity
DNase, RNase, protease, none detected
SMILES string
CN(C)CCN(C)C
InChI
1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3
InChI key
KWYHDKDOAIKMQN-UHFFFAOYSA-N
Application
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.
Preparation Note
1 of 1
This Item | |||
|---|---|---|---|
| description BioReagent, Molecular Biology, ≥99% (GC) | description BioReagent, suitable for electrophoresis, ≥99.0% | description ≥99.5%, purified by redistillation | description ReagentPlus®, 99% |
| grade Molecular Biology | grade - | grade - | grade - |
| assay ≥99% (GC) | assay ≥99.0% | assay ≥99.5% | assay 99% |
| technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| density 0.775 g/mL at 20 °C (lit.) | density 0.775 g/mL at 20 °C (lit.) | density 0.775 g/mL at 20 °C (lit.) | density 0.775 g/mL at 20 °C (lit.) |
| expl. lim. 9.08 % | expl. lim. 9.08 % | expl. lim. 9.08 % | expl. lim. 9.08 % |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B
Storage Class
3 - Flammable liquids
WGK
WGK 1
Flash Point (°F)
61.7 °F - closed cup
Flash Point (°C)
16.5 °C - closed cup
PPE (personal protective equipment)
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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