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T7409

Trypsin from porcine pancreas

lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid

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1 g
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$56.80
10 g
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$174.00
100 g
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$1,080.00

About This Item

CAS Number:
9002-07-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-650-8
MDL number:
MFCD00082094
EC Number:
3.4.21.4 (BRENDA, IUBMB)
Specific activity:
1,000-2,000 units/mg dry solid

$56.80

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type

Type II-S

Quality Segment

200

form

lyophilized powder

specific activity

1,000-2,000 units/mg dry solid

mol wt

23.8 kDa

storage temp.

−20°C

Application

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Preparation Note

This product is a lyophilized powder soluble in Hank′s Balanced Salt Solution at 25 mg/mL.

Other Notes

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. One BTEE unit = 320 ATEE units. Reaction volume = 3.2 mL (1 cm light path).
Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Disclaimer

Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.

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Trypsin from porcine pancreas lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid

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Trypsin from porcine pancreas

Trypsin from porcine pancreas Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein

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T9201

Trypsin from bovine pancreas

description

lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid

description

Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein

description

Type XI, lyophilized powder, ≥6,000 BAEE units/mg protein

description

powder, ≥7,500 BAEE units/mg solid

specific activity

1,000-2,000 units/mg dry solid

specific activity

13,000-20,000 BAEE units/mg protein

specific activity

≥6,000 BAEE units/mg protein

specific activity

≥7,500 BAEE units/mg solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

400

type

Type II-S

type

Type IX-S

type

Type XI

type

-

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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