Key Documents

View All Documentation

Trypsin from bovine pancreas

Name: Trypsin from bovine pancreas
Brand: SIGMA
Price: 67.6 USD
Availability: InStock

TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Select a Size

Change View

Size/SKU	Availability	Price
50 mg	Available to ship TODAYfromMILWAUKEE	$67.60
100 mg	Check Cart for Availability	$118.00

About This Item

CAS Number:

9002-07-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-650-8

MDL number:

MFCD00082094

EC Number:

3.4.21.4 (BRENDA, IUBMB)

Specific activity:

≥10,000 BAEE units/mg protein

Biological source:

bovine pancreas

$67.60

Available to ship TODAYDetails

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

bovine pancreas

Quality Segment

200

sterility

aseptically filled

form

essentially salt-free, lyophilized powder

specific activity

≥10,000 BAEE units/mg protein

mol wt

23.8 kDa

composition

protein, ≥95%

solubility

hydrochloric acid: soluble 1 mM, clear

foreign activity

Chymotrypsin ≤0.1 BTEE units/mg protein

storage temp.

−20°C

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Trypsin can be used to release adherent cells from tissue culture plates for passaging. Trypsin has been used in a study to assess the effects of macromolecular crowding on the structural stability of human α-lactalbumin. Trypsin has also been used in a study to investigate BN-PAGE analysis of Trichoderma harzianum secretome.

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Preparation Note

TPCK treated

Analysis Note

Protein determined by E1%/280

Other Notes

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 ml (1 cm light path).

One BAEE unit will produce a A₂₅₃ of 0.001 per minute at pH 7.6 at 25°C using BAEE as a substrate.

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	T6424	T4799	T1426
Sigma-Aldrich T8802 Trypsin from bovine pancreas	Sigma-Aldrich T6424 Trypsin from human pancreas	Sigma-Aldrich T4799 Trypsin from porcine pancreas	Sigma-Aldrich T1426 Trypsin from bovine pancreas
description TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein	description salt-free, lyophilized powder, vial of ≥1,000 BAEE units	description lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid	description TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
biological source bovine pancreas	biological source human pancreas	biological source Porcine pancreas	biological source bovine pancreas
specific activity ≥10,000 BAEE units/mg protein	specific activity -	specific activity 1,000-2,000 BAEE units/mg solid	specific activity ≥10,000 BAEE units/mg protein
form essentially salt-free, lyophilized powder	form salt-free, lyophilized powder	form lyophilized powder	form essentially salt-free, lyophilized powder
solubility hydrochloric acid: soluble 1 mM, clear	solubility -	solubility -	solubility hydrochloric acid: soluble 1 mM
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 23.8 kDa	mol wt -	mol wt 23.8 kDa	mol wt 23.8 kDa

Still not finding the right product?

Explore all of our products under Trypsin from bovine pancreas

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.