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T8823

Monoclonal Anti-T7 tag antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

Synonym(s):

T7 Tag antibody

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Size/SKUAvailabilityPrice
200 μg

Available to ship TODAYfromMILWAUKEE

$637.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
MFCD02689159
Clone:
T7 Tag
Technique(s):
western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase
Application:
WB CL
Citations:
9

$637.00

Available to ship TODAYDetails

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biological source

mouse

Quality Segment

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T7 Tag

form

buffered aqueous solution

concentration

~2 mg/mL

technique(s)

western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

General description

The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein.
The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.

Immunogen

synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH

Application

Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.

Physical form

Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

Antibody is suitable for ELISA and immunoblotting.

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Monoclonal Anti-T7 tag antibody produced in mouse purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

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Monoclonal Anti-T7 tag antibody produced in mouse

Monoclonal Anti-β-Galactosidase antibody produced in mouse clone GAL-40, ascites fluid

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Sigma-Aldrich

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IgM from bovine serum

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Sigma-Aldrich

AP130

Goat Anti-Mouse IgG & IgM Antibody

description

purified immunoglobulin, buffered aqueous solution

description

clone GAL-40, ascites fluid

description

reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution

description

Chemicon®, from goat

clone

T7 Tag

clone

GAL-40, monoclonal

clone

-

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

-

biological source

goat

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

-

antibody form

F(ab′)2 fragment of affinity isolated antibody

technique(s)

western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase

technique(s)

western blot: 1:1,000 using denatured-reduced Escherichia coli β-galactosidase, dot blot: suitable using soluble β-galactosidase, immunocytochemistry: suitable

technique(s)

-

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable, ELISA: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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