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T9028

Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse

clone TUB-1A2, ascites fluid

Synonym(s):

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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Size/SKUAvailabilityPrice
100 μL
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$831.00
0.2 mL
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$1,030.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00164753
Conjugate:
unconjugated
Clone:
TUB-1A2, monoclonal
Application:
ARR, IF, WB
Citations:
171

$831.00

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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TUB-1A2, monoclonal

contains

15 mM sodium azide

species reactivity

human, plant, animal

technique(s)

indirect immunofluorescence: 1:800 using cultured chicken fibroblasts, microarray: suitable, western blot: suitable

isotype

IgG3

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBA4A(7277), TUBB(203068)

General description

Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology.
The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus.

Immunogen

peptide containing the carboxy-terminal amino acids of α-tubulin

Application

Monoclonal Anti-Tyrosine Tubulin has been used in:
  • indirect immunofluorescent labelling
  • immunoblotting technique
  • immunocytochemical staining

Biochem/physiol Actions

The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.
Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse clone TUB-1A2, ascites fluid

Sigma-Aldrich

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Monoclonal Anti-α-Tubulin antibody produced in mouse clone DM1A, ascites fluid

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Monoclonal Anti-α-Tubulin antibody produced in mouse

Anti-β-tubulin antibody, Mouse monoclonal clone 2-28-33, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200715

Anti-β-tubulin antibody, Mouse monoclonal

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

TUB-1A2, monoclonal

clone

SAP.4G5, monoclonal

clone

DM1A, monoclonal

clone

2-28-33, monoclonal

species reactivity

human, plant, animal

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

species reactivity

dog, chicken, rat, bovine, monkey, human, mouse

Gene Information

human ... TUBA4A(7277), TUBB(203068)

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBB(203068)
mouse ... Tubb3(22152)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

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