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T9253

Trypsin inhibitor

powder

Synonym(s):

Ovomucoid

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250 mg

Available to ship TODAYfromMILWAUKEE

$73.70
500 mg

Available to ship TODAYfromMILWAUKEE

$156.00
1 g

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$238.00
5 g

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$804.00

About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-906-9
MDL number:

$73.70


Available to ship TODAYDetails


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Product Name

Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor

biological source

chicken egg white

Quality Segment

type

Type II-O

form

powder

mol wt

28 kDa

input

general use sample(s)

solubility

water: soluble 10 g/L

storage temp.

2-8°C

General description

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.
25% of ovomucoid comprises carbohydrate.

Application

Trypsin inhibitor from chicken egg white has been used:
  • to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopes
  • to treat nuclei for flow cytometric analysis
  • in Holtfreter′s solution, used for the dissociation of planarians into cells

Analysis Note

One mg will inhibit 0.8-1.6 mg of tryspin with activity of approx. 10,000 BAEE units per mg protein. May inhibit ≤0.3 mg of chymotrypsin with activity of approx. 40 BTEE units per mg protein.

Other Notes

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.
Ovomucoid, from chicken egg white, does not itself inhibit chymotrypsin. Chymotrypsin inhibition is a measure of ovoinhibitor contamination by method of Feeney, R. E., et al., J. Biol. Chem., 238, 1415 (1963).
View more information on Trypsin Inhibitor.

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This Item
T2011A7641A9207
description

Type II-O, Partially purified ovomucoid, containing ovoinhibitor

description

Type III-O (free of ovoinhibitor)

description

lyophilized powder

description

aqueous glycerol suspension

biological source

chicken egg white

biological source

chicken egg white

biological source

chicken egg white

biological source

egg white

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

powder

form

powder

form

lyophilized powder

form

aqueous glycerol suspension

solubility

water: soluble 10 g/L

solubility

water: soluble 10 g/L

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

28 kDa

mol wt

27 kDa

mol wt

44287 Da by calculation

mol wt

-


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Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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Questions

  1. At what temperature should aliquots be stored at?

    1 answer
    1. Aliquots of this product should be stored frozen at -20 °C.

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