The microprotein kit TP0200 is not intended for use above 1 mg/ml
TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
Synonym(s):
Total Protein Assay Kit
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Available to ship TODAYfromMILWAUKEE | $145.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
$145.00
Available to ship TODAYDetails
description
wavelength 500-750nm
Quality Level
usage
sufficient for ~50 manual assays
protein detection level measuring range
0-1 mg/mL
parameter
18-26 °C temperature, 200 μL sample volume
storage temp.
2-8°C
General description
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Application
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins[1]
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)[2]
- in the stored samples of rat liver microsomal fractions
Biochem/physiol Actions
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| protein detection level measuring range 0-1 mg/mL | protein detection level measuring range - | protein detection level measuring range - | protein detection level measuring range - |
| description wavelength 500-750nm | description - | description - | description - |
| usage sufficient for ~50 manual assays | usage sufficient for ~200 assays | usage - | usage - |
| storage temp. 2-8°C | storage temp. - | storage temp. room temp | storage temp. 2-8°C |
| parameter 18-26 °C temperature | parameter - | parameter - | parameter - |
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Instructions
Jose Laparra et al.
Oncotarget, 10(7), 760-772 (2019-02-19)
Imbalances in innate immunity and the activity of innate immune cells are implicated in the development of hepatocellular carcinoma (HCC). Plant seeds are good sources of protease inhibitors, which can have a significant influence on human health disorders, especially in
A Smialowska et al.
Journal of dairy science, 100(4), 2553-2563 (2017-02-22)
We isolated goat phosphopeptides via calcium and ethanol precipitation from a caseinate digest and investigated their feasibility as an iron-fortification ingredient in nutritional foods. Goat tryptic-digested phosphopeptides could bind 54.37 ± 0.50 mg of Fe/g of protein compared with goat
David Kessel
Photochemistry and photobiology, 90(5), 1211-1213 (2014-04-26)
When the initial effect of photodynamic therapy (PDT) involves mitochondrial photodamage, an early effect is loss of the mitochondrial membrane potential (ΔΨm ). Using murine hepatoma 1c1c7 cells and a photosensitizing agent known to target mitochondria, we examined loss of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| TP0200-1KT | 04061837398001 |
-
does it works for samples with 11 mg/ml?
1 answer-
Helpful?
-