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TRUE6

TrueGel3D Hydrogel Kits

SLO-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker

Sinonimo/i:

3D Hydrogel Kit, TrueGel3D Kit

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1 kit
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R$ 632,00

Informazioni su questo articolo

NACRES:
NA.71
UNSPSC Code:
12352207
Storage temp.:
−70°C
Shipped in:
dry ice

R$ 632,00


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shipped in

dry ice

storage temp.

−70°C

General description

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-DEXTRAN does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

TRUE6-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic natural tissue stiffness and to investigate the effect of mechanical stimulus on cell physiology and behavior. It is also used to culture non-proliferating cells and proliferating spheroids that do not attach to hydrogel for survival, but rather use their own extracellular matrix.

Features and Benefits

  • Slow gelling (takes 8 minutes to 25 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

Other Notes

TRUE6-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

Disclaimer

Avoid prolong exposure of PEG non cell-degradable crosslinker to air.

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storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice


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Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

wgk

WGK 3

Classe di stoccaggio

10 - Combustible liquids



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Protocolli

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

Articoli

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

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Numero articolo commerciale globale

SKUGTIN
TRUE6-1KT04061835539291

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