This product has not been tested for cellulase activity. However, this material is a product of Novozymes and is commercially available as Pentopan Mono BG. Some literature indicate cellulase activity in this preparation is negligible. Please see the link below to review a publication that determined that cellulase activity was not detected.
https://www.sciencedirect.com/science/article/pii/S0926669022001388
X2753
Xylanase
powder, ≥2500 units/g, recombinant, expressed in Aspergillus oryzae
Greener Alternative
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| Size/SKU | Availability | Price |
|---|---|---|
10 g | Available to ship TODAYfromMILWAUKEE | $130.00 |
50 g | Available to ship TODAYfromMILWAUKEE | $332.00 |
About This Item
Specific activity:
≥2500 units/g
Recombinant:
expressed in Aspergillus oryzae
recombinant
expressed in Aspergillus oryzae
Quality Segment
form
powder
specific activity
≥2500 units/g
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
storage temp.
2-8°C
General description
Studies show that the signal sequence found in the xylanase gene is critical for protein production in E. coli. Loss of this sequence resulted in a significant reduction in expression.
Xylanase catalyses the degradation of xylan into xylooligosaccharides and D-xylose. It also breaks down hemicellulose. Xylanase is used for the production of biofuels, such as ethanol and xylitol. It improves the quality of bread and juices. Xylanase is used in biobleaching process and decreases environmental pollution.
Purified endo-β−(1→4)-xylanase from Thermomyces lanuginosus produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research
Application
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
A product of Novozyme Corp.
1 of 1
This Item | |||
|---|---|---|---|
| description powder, ≥2500 units/g, recombinant, expressed in Aspergillus oryzae | description lyophilized powder, ≥100 units/mg protein | description - | description lyophilized powder, ≥10 units/mg protein |
| specific activity ≥2500 units/g | specific activity ≥100 units/mg protein | specific activity ≥10 KLU/g | specific activity ≥10 units/mg protein |
| recombinant expressed in Aspergillus oryzae | recombinant - | recombinant expressed in Aspergillus oryzae | recombinant - |
| form powder | form lyophilized powder | form liquid | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| greener alternative category | greener alternative category , Enabling | greener alternative category - | greener alternative category - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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is this xylanase cellulase-free?
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该产品中酶的分子量是多少
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The molecular weight of this enzyme is approximately 24 kDa.
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Will this enzyme in buffer be deactivated completely at 100°C boiling for 10 mins towards end of protocol or it needs acid treatment strictly ?
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We have not tested boiling this enzyme at the end of the protocol, we have only used acid.
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Is this enzyme powder soluble in water ? seems like particle dispersing in buffer at Ph 4.8 ?
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The active components of xylanase X2753 are readily soluble in water at concentrations that occur in normal usage. However, water solutions will be turbid because of the wheat flour used for standardization of the enzyme.
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What does >2500U/g imply. What is range of enzyme activity for this enzyme under your protocols kindly inform.
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Each Lot is guaranteed to have a specific activity greater than or equal to 2500 Units/g. There is no maximal value specified.
The calculated specific activity for each lot will be printed on the Certificate of Analysis found here:
https://www.sigmaaldrich.com/product/sigma/x2753#product-documentationHelpful?
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What is the optimal pH, buffer, temperature and concentration of this product ?
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This is a product of Novozymes Corp. The manufacturer tests this material according to the following protocol and conditions:
Temperature - 50°C
Buffer - 0.1M Phosphate Buffer, pH 6
Enzyme concentration - 0.007 - 0.057 units/mL
Substrate - 0.45%w/v Azo-Wheat arabinxylan in 0.1 M Phosphate Buffer, pH 6
Reaction - 0.100 mL enzyme solution added to 0.900 mL substrate solution
Incubation time - 30 minutes in a water bath at 50°C
Stop Reaction - add 5 mL of Stop Reagent: 6.66 mL 2 N HCl in 1000mL absolute Ethanol
Incubation - 15 - 60 minutes at ambient temperature
Centrifuge at 4000 rmp for 15 minutes
Detection - 585 nm (manual) within 20 minutes of centrifugationHelpful?
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We have Sigma X2753 Xylanase We need to treat our sample with this. Kindly suggest a protocol at which this product will show its optimum activity.
1 answer-
There is not a protocol offered specifically for this product. However, the peer-reviewed articles may be helpful:
https://www.sigmaaldrich.com/search/x2753?focus=papers&page=1&perpage=30&sort=relevance&term=x2753&type=citation_searchHelpful?
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What is the density of Product X2753, Cellulase from Aspergillus oryzae?
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We have not determined the density of this product.
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What is the optimum pH for Cellulase from Aspergillus oryzae., Product X2753?
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Product X2753, Xylanase, has activity in the pH range of 4-6, and tolerance with temperatures up to 75 °C. The following article may be helpful: https://www.sciencedirect.com/science/article/pii/S138917230670603X#aep-section-id10
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