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REDExtract-N-Amp^™ Blood PCR Kit

Name: REDExtract-N-Amp<SUP>™</SUP> Blood PCR Kit
Brand: SIGMA
Price: 344 USD
Availability: InStock

sufficient for 100 extractions, sufficient for 100 amplifications

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1 kit	Estimated to ship onOctober 01, 2026fromSAINT LOUIS	$344.00

About This Item

NACRES:

NA.55

UNSPSC Code:

41106303

$344.00

Estimated to ship onOctober 01, 2026Details

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Properties

Quality Segment

200

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

feature

dNTPs included, hotstart, Direct PCR

technique(s)

PCR: suitable, whole genome amplification: suitable

color

red

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

Description

General description

The REDExtract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration, or alcohol precipitation. The kit also includes a PCR ReadyMix^™, that uses an antibody based hot start and is specially formulated for amplification directly from the extract. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Genomic DNA is extracted from 10 μL of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR Mix.

The REDExtract-N-Amp^™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp^™ Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, endocervical swab eluates of mice and parasites. It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.

Biochem/physiol Actions

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Features and Benefits

Efficient 8 minute prep allows greater speed and throughput
No need for organic extraction, centrifugation or alcohol precipitation
Hot start antibody for highly specific PCR amplification of genomic DNA
Can be used with whole blood or blood cards
Extract stable at 4 °C for at least 6 months
Compatible with any format (single tube, 96-well, etc.)
Simple, 3 step procedure with no special equipment required
REDExtract-N-Amp^™ requires no loading buffers or tracking dyes required for gel analysis
PCR products are suitable for direct cloning and sequencing

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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This Item	XNABE	XNABS	XNABR
Sigma-Aldrich XNAB REDExtract-N-Amp^™ Blood PCR Kit	Sigma-Aldrich XNABE REDExtract-N-Amp^™ Blood PCR Kit	Sigma-Aldrich XNABS REDExtract-N-Amp^™ Blood PCR Kit	Sigma-Aldrich XNABR REDExtract-N-Amp^™ Blood PCR Kit
description sufficient for 100 extractions, sufficient for 100 amplifications	description sufficient for 100 extractions, sufficient for 500 amplifications	description sufficient for 10 extractions, sufficient for 10 amplifications	description sufficient for 1000 extractions, sufficient for 1000 amplifications
technique(s) whole genome amplification: suitable, PCR: suitable	technique(s) PCR: suitable	technique(s) PCR: suitable, whole genome amplification: suitable	technique(s) whole genome amplification: suitable, PCR: suitable
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
feature hotstart, Direct PCR, dNTPs included	feature hotstart, Direct PCR, dNTPs included	feature Direct PCR, hotstart, dNTPs included	feature hotstart, dNTPs included, Direct PCR
input crude DNA	input crude DNA	input crude DNA	input crude DNA
usage sufficient for 100 extractions, sufficient for 100 reactions, sufficient for 100 amplifications	usage sufficient for 500 amplifications, sufficient for 500 reactions, sufficient for 100 extractions	usage sufficient for 10 reactions, sufficient for 10 extractions, sufficient for 10 amplifications	usage sufficient for 1000 reactions, sufficient for 1000 extractions, sufficient for 1000 amplifications

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Kit Components Also Available Separately

Product No.

Description

SDS & Pricing

L3289
Lysis Solution for Blood
SDS

N9784
Neutralization Solution for Blood
SDS

P8240
REDExtract-N-Amp^™ PCR ReadyMix^™ for Blood
SDS

Pictograms

GHS05

Signal Word

Danger

Hazard Codes

H290,H314

Precautionary Statements

P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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