Yes, this yeast drop-out medium supplement can be autoclaved. Please see the datasheet for additional preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/255/001/y2021dat.pdf
Y2021
Yeast Synthetic Drop-out Medium Supplements
without histidine, leucine, tryptophan, and adenine
Synonym(s):
Yeast Synthetic Drop-out Media Supplements
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| Size/SKU | Availability | Price |
|---|---|---|
20 g | Available to ship TODAY | $220.00 |
About This Item
$220.00
Available to ship TODAYDetails
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Quality Segment
form
powder
technique(s)
molecular cloning: suitable, transformation: suitable
application(s)
microbiology
storage temp.
room temp
Application
Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Preparation Note
Dissolve 1.39 g of supplement per liter of medium.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
1 of 1
This Item | |||
|---|---|---|---|
| description without histidine, leucine, tryptophan, and adenine | description without uracil, leucine, and tryptophan | description without histidine, leucine, and tryptophan | description without lysine |
| form powder | form powder | form powder | form powder |
| application(s) microbiology | application(s) microbiology | application(s) microbiology | application(s) - |
| technique(s) transformation: suitable, molecular cloning: suitable | technique(s) molecular cloning: suitable, transformation: suitable | technique(s) molecular cloning: suitable, transformation: suitable | technique(s) molecular cloning: suitable |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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can I autoclave this particular yeast dropout medium supplement?
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