This product, in suspension, is stable for up to 1 month at 0–4°C. The long term stability, when stored at 20°C or 80°C, has not been determined. However, other sources indicate prepared suspensions are stable for 1 month at 20°C and for 6 months at 80°C. This information has not been validated. As a general practice, store in aliquots to avoid repeated freeze/thaw cycles. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/925/825/z4250pis.pdf
Z4250
Zymosan A from Saccharomyces cerevisiae
for inducing inflamatory response
Synonym(s):
Yeast Cell Wall
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| Size/SKU | Availability | Price |
|---|---|---|
250 mg | Check Cart for Availability | $77.40 |
1 g | Available to ship TODAYfromMILWAUKEE | $228.00 |
5 g | Available to ship TODAYfromMILWAUKEE | $949.00 |
About This Item
Form:
powder with small lumps
Biological source:
Saccharomyces cerevisiae
biological source
Saccharomyces cerevisiae
Quality Segment
form
powder with small lumps
technique(s)
flow cytometry: suitable, tissue culture: suitable
color
off-white to light brown
storage temp.
2-8°C
Application
- in flow cytometry
- to study its effect on head kidney leukocytes from rainbow trout (Oncorhynchus mykiss)
Zymosan A from Saccharomyces cerevisiae has been used:
- as a positive control to determine the activation of complement cascade
- for respiratory burst (RB) assay
- to assess its effects on performance, immunity, heat shock protein and apoptosis related gene expressions of dairy cows under heat stress (HS)
- a stimulating agent in Krebs-Ringer phosphate (KRP) medium
- to induce thermal hyperalgesia in mice
Biochem/physiol Actions
Zymosan is prepared from yeast cell wall and consists of protein-carbohydrate complexes. It is used to induce experimental sterile inflammation. In macrophages, zymosan-induced responses include the induction of proinflammatory cytokines, arachidonate mobilization, protein phosphorylation, and inositol phosphate formation. Zymosan A also raises cyclin D2 levels suggesting a role for the latter in macrophage activation besides proliferation. It potentiates acute liver damage after galactosamine injection suggesting that certain types of nonparenchymal cells other than Kupffer cells are involved in zymosan action. Zymosan A, a 13 beta glucan contributes to the development of organic dust-induced respiratory disease.
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This Item | |||
|---|---|---|---|
| description for inducing inflamatory response | description Type II | description A major structural element of the yeast cell wall having a β1,3-glucan linear structure with a small number of β1,6-glucan branches. Has been shown to induce an oxidative burst response from NR8383 cells. | description Type I |
| biological source Saccharomyces cerevisiae | biological source Saccharomyces cerevisiae | biological source - | biological source Saccharomyces cerevisiae |
| technique(s) flow cytometry: suitable, tissue culture: suitable | technique(s) - | technique(s) - | technique(s) - |
| form powder with small lumps | form powder or solid | form solid | form powder or solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| color off-white to light brown | color - | color off-white | color - |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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If I want to solubilize this product and store it for long-term use afterward, can I also store it at -80 °C?
1 answer-
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How can I solubilize Zymosan? The information sheet does not provide this information. Can it be dissolved in water?
1 answer-
This product is soluble in organic solvents as follows:
- Chloroform: 100 µg/ml
- Dimethyl sulfoxide: 200 µg/ml
- Ethanol: 100 µg/ml.According to the 'Solubility/Solution Stability' section in the product information sheet, this product is not soluble in water but can be suspended uniformly in saline solution.
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Is Z4250, Zymosan A from Saccharomyces cerevisiae, soluble in DMSO?
1 answer-
Yes, this item is soluble in the following solvents:
- Chloroform: 100 µg/ml
- DMSO: 200 µg/ml
- Ethanol: 100 µg/mlHelpful?
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What is the appearance of Product Z4250, Zymosan?
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The product is a powder which is described on the specification sheet.
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What is the source of the Zymosan in Product Z4250?
1 answer-
This zymosan is prepared from single cell yeast.
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What is the particle size of Product Z4250, Zymosan?
1 answer-
Sigma-Aldrich does not measure the particle size of zymosan. The average diameter of zymosan particles has been reported to be about 3 μm, as described on the product information sheet (under Documents, above), with the cited reference.
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Can Product Z4250, Zymosan suspension be autoclaved?
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We have no information as to whether zymosan suspensions can be autoclaved. However, suspensions are stable to boiling conditions.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can Product Z4250, Zymosan be prepared for use in a properdin assay?
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Information on preparation of zymosan for use in a properdin assay, which includes boiling zymosan suspensions, is described on the product information sheet (under Documents, above).
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How can I solubilize Zymosan, Product Z4250?
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Solubility information can be found in the product information sheet (under Documents, above) on our website.
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