Ascentis^® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Column

Yes, we have guard columns available for product 53323-U Ascentis® Express Peptide ES C18 HPLC Column.  Product 53500-U is the Ascentis® Express Guard Holder and Product 53542-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 4.6 mm .

Product 53323-U Ascentis® Express Peptide ES C18 HPLC Column provides a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for product 53323-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.

This product 53323-U Ascentis® Express Peptide ES C18 HPLC Column is stable to operating pressures up to 600 bar (9000 psi)

Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53323-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

Nothing special is required to use product 53323-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).

The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of product 53323-U Ascentis® Express Peptide ES C18 HPLC Column for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

Yes.  product 53323-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, product 53323-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53323-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.