HPLC Analysis of Flavonoids in Lime Juice on Ascentis® Express C18

HPLC Analysis of Flavonoids in Lime Juice on Ascentis® Express C18


sample preparation SPE (Solid Phase Extraction)
sample/matrix lime juice hand-squeezed in laboratory, centrifuged and filtered through a 0.45 µm nylon filter disc (Acrodisc)
column Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 µm particles (53826-U)
column temp. ambient
mobile phase [A] water/formic acid (99.9:0.1); [B] water/acetonitrile/isopropanol/formic acid (39.9:20:40:0.1)
gradient 10% B for 3 min; 10 to 34% B in 30 min; 34 to 100% B in 1 min; held at 100% B for 6 min; 100 to10% B in 1 min
flow rate 0.8 mL/min
injection 2 µL
detector ESI-MS: mass spectral range, 250-700 m/z; interval, 0.5 s; scan speed, 938 amu/s; nebulizing gas (N2) flow, 1.5 L/min; (interface temperature, 350 °C; heat block, 300 °C; desolvation line (DL) temperature, 300 °C; DL voltage, 34 V; probe voltage, +4.5 kV; Qarray voltage, 1.0 V; RF voltage, 90 V; detection gain, 1.05 kV)
detector PDA: 190-370 nm; chromatogram extracted at 283 nm


Analysis Note Flavonoids are polyphenolic compounds ubiquitous in nature and classified, according to their chemical structure, into flavonols, flavones, flavanones, isoflavones, catechins, anthocyanidins and chalcones. They are widely present in fruits, vegetables, leaves, nuts, seeds, bark and beverages (tea, coffee, beer, wine and fruit drinks).
Categories Analytical Chromatography, Food & Beverage Analysis, SPE Applications, Flavors, Fragrances, Beverage Testing, Flavors/fragrances, Beer, Wine, and Spirits, Fruit Juice, Wine, Beer
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC


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