Supel™-Select Polymeric SPE
HLB and Ion-Exchange phases for a wide range of applications and pH conditions
Supel™-Select SPE phases are ideal for the solid phase extraction of a broad range of compounds from aqueous samples. While reversed-phase interactions dominate retention on the Supel™-Select HLB, and the retention mechanisms of the Supel™-Select SAX and SCX are predominately based on ion-exchange, the hydrophilic modifications of the styrene-based polymer backbone allow for retention and recovery of more polar compounds.
| HLB Phase Chemistry: | Hydrophilic modified styrene polymer |
| SAX Phase Chemistry: | Quaternary amine functionalized hydrophilic modified styrene polymer |
| SCX Phase Chemistry: | Sulfonic acid functionalized hydrophilic modified styrene polymer |
| pH Compatibility: | 0-14 |
| Particle Size: | 50-70 µM |
| MS Suitability: | Yes |
| Surface Area: | 160-420 m2/g |
| Pore Volume: | 0.8-1.2 mL/g |
| Pore Size: | 80-200 Å |
| Feature | Benefit |
|---|---|
| Hydrophilic modified styrene resin | Extract and recover a broad range of analytes (polar to non-polar, acidic to basic) from aqueous samples |
| Method simplicity | Save time, money, and frustration during method development |
| Greater capacity | Allows for smaller bed weights = smaller elution volumes = time savings in sample processing |
| Resistant to overdrying | More robust methodology and better reproducibility |
| Low UV and MS extractables | Lower background for greater sensitivity |
| Stringent production & QC | Greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision |
High and Reproducible Recoveries
The hydrophilic, lipophilic balanced Supel™-Select HLB SPE allows users to extract a broad range of compounds using a single sorbent and generic methodology. Analyte recovery was high across all the compounds tested, and results were highly reproducible across three production lots.
Minimal Extractables
Supel™-Select HLB SPE phases are quality controlled for both LC-UV and LC-MS/MS extractables. As assays require greater sensitivity, analytical “cleanliness” of both SPE phases and associated hardware becomes more critical.
In this simple test, acetonitrile was passed through both Supel™-Select HLB SPE tubes and the leading competitor’s tubes followed by LC-UV analysis.
Supel™-Select HLB SPE imparted less than 25% of UV extractables than the leading competitor.
Figure 1.
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