Restriction Enzyme Buffer Reference

Our restriction enzyme collection has been optimized for digestion using five unique buffers. Reference the table below for the enzyme activity in each buffer.

 

Table 1
Activity (%) of each restriction enzyme in each buffer. The preferred buffer for each enzyme is highlighted in bold.
 

Buffers
Restriction Enzyme   SuRE/
Cut Buffer A
SuRE/Cut
Buffer B
SuRE/Cut
Buffer L
SuRE/Cut
Buffer M
SuRE/
Cut Buffer H
10899208001 Apa I 100 10-25 50-75 50-75 0-10
11558170001 BLN I 25-50 50-75 0-10 25-50 100
10742988001 DPN I 100 75-100 50-75 75-100 75-100
10822248001 MAE III* 0-10 10-25 0-10 0-10 10-25
10835323001 NCO I 50-75 50-75 50-75 50-75 100
10885851001 NHE I 100 25-50 100 100 10-25
11014714001 NOT I 25-Oct 50-75 0-10 25-50 100
10650129001 PVU I 50-75 75-100 25-50 50-75 100
10669792001 SAC I 100 0-10 100 50-75 0-10
11288024001 SFI I 25-50 25-50 75-100 100 25-50
11047639001 SMA I 100 0-10 0-10 0-10 0-10
11207644001 SPE I 75-100 75-100 75-100 100 100
11047663001 XBA I 100 75-100 75-100 75-100 100