616370-M

Aprotinin, Bovine Lung, Crystalline

Aprotinin, Bovine Lung, Crystalline, CAS 9087-70-1, is a competitive, reversible, heat- and acid-stable inhibitor of proteolytic and esterolytic activities.

• Sinônimo(s): Aprotinin, Bovine Lung, Crystalline, Pancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor
• Número CAS: 9087-70-1
• Número MDL: MFCD00130541
• Código UNSPSC: 12352202
• NACRES: NA.25

Propriedades

• Nível de qualidade: 200
• descrição: Merck USA index - 14, 757
• Ensaio: ≥95% (gel filtration chromatography)
• Formulário: crystalline solid
• atividade específica: ≥5500 KIU/mg dry wt
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic)
• Impurezas: ≤10 EU/mg endotoxin
• cor: white to off-white
• pI: 10.5
• solubilidade: water: 10 mg/mL
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O
• InChI: 1S/C284H432N84O79S7/c1-21-144(9)222-271(439)337-174(68-46-105-309-282(300)301)239(407)340-187(120-160-77-85-164(374)86-78-160)251(419)341-185(116-156-55-29-24-30-56-156)250(418)342-188(121-161-79-87-165(375)88-80-161)252(420)346-191(123-208(291)378)246(41
• chave InChI: ZPNFWUPYTFPOJU-UHFFFAOYSA-N

Descrição

Descrição geral

A competitive and reversible inhibitor of esterase and protease activity. Forms a tight complex with and blocks the active site of target enzymes. Inhibits a number of different proteases, including chymotrypsin, coagulation factors involved in the pre-phase of blood clotting, kallikrein (K_d = 1 X 10^-7 M), plasmin (K_d = 2.3 X 10^-10 M), tissue and leukocyte proteinases, and trypsin (K_d = 5 X 10^-14 M). Does not inhibit Factor Xa or thrombin. Reported to be relatively acid and heat stable. Effectively inhibits target proteases at equimolar concentration. Useful as a serine proteinase inhibitor during purification of proteins and in studies of zymogen activation systems. Reported to suppress virus HA cleavage and limit reproduction of human and avian influenza viruses with a single arginine in the HA cleavage site.

Effectively inhibits target proteases at equimolar concentration.

Note: 1 KIU = 0.025 antiplasmin units (APU) = 0.0016 trypsin inhibitor units (using porcine trypsin) = 0.0009756 trypsin inhibitor units (using bovine trypsin).

Ações bioquímicas/fisiológicas

Kd = 1 x 10-7 M, 2.3 x 10-10 M, 5 x 10-14 M, against kallikrein, plasmin, and trypsin, respectively

Primary Target
chymotrypsin

Nota de preparo

Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

Outras notas

One Kallikrein Inhibitory Unit (K.I.U.) is defined as the quantity of protease inhibitor that will inhibit two kallikrein units by 50% under optimal conditions. Note: 1 KIU = 0.025 antiplasmin units (APU) or 0.0031 trypsin inhibitor units.

Zhirnov, O.P., et al. 2011. Antiviral Res.92, 27.
Roberts, R.F., et al. 1998. Ann. Thorac. Surg.66, 225.
Azougagh Oualane, F., et al. 1992. Thromb. Res.68, 185.
Anderson, L.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 2979.
Kruithof, W.L., et al. 1986. Biochem. J.226, 631.
McKeehan, W.L., et al. 1986. J. Biol. Chem.261, 5378.
Fritz, H., and Wunderer, G. 1983. Arzneim. Forsch.33, 479.
Rijken, D.C., and Collen, D. 1981. J. Biol. Chem.256, 7035.
Kassell, B., et al. 1970. Methods Enzymol.19, 845.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Toxicity: Standard Handling (A)

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable