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Merck

MAB2510

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2

clone 264-449-2, Chemicon®, from mouse

Sinônimo(s):

GFP

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Sobre este item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

264-449-2, monoclonal

reatividade da espécie (prevista por homologia)

all

embalagem

antibody small pack of 25 μg

fabricante/nome comercial

Chemicon®

técnica(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

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Este Item
G6539G6795AB3080
biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

264-449-2, monoclonal

clone

GFP-20, monoclonal

clone

GSN24, monoclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C, 2-8°C

storage temp.

-

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Descrição geral

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Imunogênio

Epitope: original GFP only
highly-purified native GFP

Aplicação

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2 is an antibody against Green Fluorescent Protein for use in IP, WB & IC.
Immunocytochemistry

Immunoblotting: 1:1000

Immunoprecipitation

Optimal working dilutions and protocols must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Ações bioquímicas/fisiológicas

Directed against a bacterially-expressed fusion protein. Also reacts with red-shifted GFP-variant. Does not react with enhanced GFP.

forma física

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Nota de preparo

Store at 2-8°C for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Nota de análise

Tested

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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The neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with TAR DNA-binding protein-43 (TDP-43) inclusions (FTLD-TDP) share the neuropathological hallmark of aggregates of TDP-43. However, factors governing the severity and regional distribution of TDP-43 pathology, which may account
Guoqiang Cai et al.
The FEBS journal, 272(7), 1625-1638 (2005-03-30)
Neurotransmitter transporters play a major role in achieving low concentrations of their respective transmitter in the synaptic cleft. The GABA transporter GAT1 belongs to the family of Na(+)- and Cl(-)-coupled transport proteins which possess 12 putative transmembrane domains and three
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PloS one, 12(9), e0185498-e0185498 (2017-09-28)
Diabetes is a chronic disease characterized by high levels of blood glucose. Diabetic patients should normalize these levels in order to avoid short and long term clinical complications. Presently, blood glucose monitoring is dependent on frequent finger pricking and enzyme
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Redox biology, 32, 101458-101458 (2020-03-08)
Spinocerebellar ataxia type-1 (SCA1) is caused by an abnormally expanded polyglutamine (polyQ) tract in ataxin-1. These expansions are responsible for protein misfolding and self-assembly into intranuclear inclusion bodies (IIBs) that are somehow linked to neuronal death. However, owing to lack
S Matsuda et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 21(17), 6597-6607 (2001-08-23)
Using a yeast two-hybrid method, we searched for amyloid precursor protein (APP)-interacting molecules by screening mouse and human brain libraries. In addition to known interacting proteins containing a phosphotyrosine-interaction-domain (PID)-Fe65, Fe65L, Fe65L2, X11, and mDab1, we identified, as a novel

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