It is not possible to have cells that are PI positive without the cells also being positive for Annexin V binding. Cells are PI positive because the membrane has been compromised. If this is the case, Annexin V can also enter the cell and bind to the PS on the internal cell membrane. The gating on the histogram for the FITC channel should be changed so that all cells that are PI positive are also Annexin V positive.
APOAF
Kit de detecção de apoptose com anexina V com FITC
Sinônimo(s):
Annexin V-FITC, Apoptosis probe FITC
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Selecione um tamanho
20 TEST
R$ 1.791,00
60 TEST
R$ 6.289,00
R$ 1.791,00
Previsão de entrega em08 de janeiro de 2026Detalhes
Sobre este item
NACRES:
NA.84
UNSPSC Code:
12352207
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(20 tests)
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Quality Level
1 of 4
Este Item | APOAC | S7111 | QIA39 |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) flow cytometry: suitable |
| usage (20 tests) | usage sufficient for 200 tests | usage - | usage sufficient for 50 tests |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging - |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) - |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
Application
O kit de detecção de apoptose com anexina V com FITC foi utilizado:
- na coloração de células neoplásicas de próstata LNCaP para medir a atividade de extratos de G. lucidum durante o tratamento do câncer de próstata.[1]
- para marcação de células tumorais para estudar a atividade inibitória de DBP-maf (proteína ligada à vitamina D-fator de ativação de macrófagos) em células de tumor de próstata.[2]
- para medição indireta da atividade de flipase.[3]
Features and Benefits
Detecta apoptose antes no processo que os ensaios à base de DNA, como TUNEL.
- Marcação rápida das células. A coloração das células leva apenas 10 minutos.
- Não é necessário fixar ou processar as células, reduzindo o tempo de detecção e permitindo que as células sejam usadas em estudos posteriores.
- O corante secundário de iodeto de propídio está incluído no kit para diferenciar células apoptóticas de células viáveis e necróticas.
General description
O kit de anexina V com FITC permite a detecção fluorescente da anexina V ligada a células apoptóticas e a determinação quantitativa por citometria de fluxo. O kit de anexina V com FITC usa anexina V conjugada com isotiocianato de fluoresceína (FITC) para marcar os sítios de fosfatidilserina na superfície da membrana. O kit inclui iodeto de propídio (IP) para marcar o DNA celular em células necróticas em que a membrana celular foi totalmente danificada. Essa combinação permite a diferenciação entre as células apoptóticas prematuras (positivas para anexina V positivo, negativas para IP), as células necróticas (positivas para anexina V, positivas para IP) e as células viáveis (negativas para anexina V, negativas para IP).
Other Notes
Permita que todos os componentes atinjam a temperatura ambiente antes do uso.
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10 - Combustible liquids
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Conteúdo relacionado
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When using Product APOAF, Annexin V-FITC Apoptosis Detection Kit, I have cells that are Annexin V FITC negative and PI positive. What are these cells?
1 answer-
Helpful?
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Can I use Product APOAF, Annexin V-FITC Apoptosis Detection Kit, to differentiate cells that are dead due to necrosis or apoptosis?
1 answer-
When using a kinetic study (various time points), you can show the progression of the cells from viable (annexin V FITC negative, PI negative), to annexin FITC positive, PI negative (membrane flip) to annexin V FITC positive, PI positive (dead). If there are cells that are double positive when starting, it is not possible to guarantee that the cell death occurred due to apoptosis with this assay.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product APOAF, Annexin V-FITC Apoptosis Detection Kit be used on fixed cells?
1 answer-
No. Product APOAF, Annexin V-FITC Apoptosis Detection Kit must be performed on live cells in order to measure Apoptosis. The assay is based on the externization of phosphatidylserine from the inner cell membrane to the outer cell membrane. If the membrane is preturbed due to fixation, non-speciifc staining of the inner cell membrane might occur
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When using the Annexin V-FITC Apoptosis Detection Kit, Product APOAF, can I use any buffer for resuspending my cells?
1 answer-
The binding buffer included in the kit needs to be used for resuspending cells. The buffer contains calcium chloride at a final (1X) concentration of 2.5 mM which is necessary for the binding of annexin V to phosphatidylserine.
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What wavelength do I use to detect Annexin V-FITC and Propidium Iodide when using Annexin V-FITC Apoptosis Detection Kit, Product APOAF?
1 answer-
Annexin V FITC will have a maximum emission of 528 nm. This can be measured in the standard FITC Channel on a flow cytometer (FL1). Propidium Iodide has a maximum emission of 620 nm. This is measured on the short red channel on a flow cytometer (FL2 or FL3).
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