E7764

Endothelin 1

≥97% (HPLC), powder

• Synonym(s): ET-1
• Empirical Formula (Hill Notation): C₁₀₉H₁₅₉N₂₅O₃₂S₅
• CAS Number: 117399-94-7
• Molecular Weight: 2491.90
• Número MDL: MFCD00133305
• ID de substância PubChem: 24894656

Properties

• fonte biológica: human
• Nível de qualidade: 200
• teor: ≥97% (HPLC)
• forma: powder
• technique(s): ELISA: suitable
• cor: white
• solubilidade: 1% acetic acid: >1 mg/mL; H₂O: >1 mg/mL
• nº de adesão UniProt: P05305
• temperatura de armazenamento: −20°C
• SMILES string: [H]N[C@H]1CSSC[C@H](NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](Cc3ccc(O)cc3)NC(=O)[C@@H](NC(=O)[C@@H]4CSSC[C@H](NC(=O)[C@H](CO)NC1=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N4)C(C)C)C(=O)N[C@@H](Cc5cnc[nH]5)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc6c[nH]c7ccccc67)C(O)=O
• InChI: 1S/C109H159N25O32S5/c1-12-56(9)87(107(163)125-76(109(165)166)39-60-43-113-65-24-18-17-23-63(60)65)134-108(164)88(57(10)13-2)133-99(155)75(42-85(143)144)123-94(150)70(36-54(5)6)118-97(153)73(40-61-44-112-52-114-61)121-103(159)80-49-169-168-48-64(111)89(145)126-77(45-135)102(158)131-81-50-170-171-51-82(105(161)132-86(55(7)8)106(162)124-72(38-59-26-28-62(138)29-27-59)95(151)120-71(96(152)130-80)37-58-21-15-14-16-22-58)129-91(147)67(30-31-83(139)140)116-90(146)66(25-19-20-33-110)115-98(154)74(41-84(141)142)122-92(148)68(32-34-167-11)117-93(149)69(35-53(3)4)119-100(156)78(46-136)127-101(157)79(47-137)128-104(81)160/h14-18,21-24,26-29,43-44,52-57,64,66-82,86-88,113,135-138H,12-13,19-20,25,30-42,45-51,110-111H2,1-11H3,(H,112,114)(H,115,154)(H,116,146)(H,117,149)(H,118,153)(H,119,156)(H,120,151)(H,121,159)(H,122,148)(H,123,150)(H,124,162)(H,125,163)(H,126,145)(H,127,157)(H,128,160)(H,129,147)(H,130,152)(H,131,158)(H,132,161)(H,133,155)(H,134,164)(H,139,140)(H,141,142)(H,143,144)(H,165,166)/t56-,57-,64-,66-,67-,68-,69-,70-,71-,72-,73-,74-,75-,76-,77-,78-,79-,80-,81-,82-,86-,87-,88-/m0/s1
• InChI key: ZUBDGKVDJUIMQQ-ZTNLKOGPSA-N
• Gene Information: human ... EDN1(1906)

Description

Amino Acid Sequence

Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp [Disulfide Bridges: 1-15; 3-11]

Descrição geral

Endothelin-1 (ET-1) is synthesized and secreted by activated endothelial cells, and is a peptide with potent vasoconstrictor activity. It is a marker for activated or dysfunctional endothelial cells. This peptide is one of the three isopeptides belonging to the endothelin family. It is also released by cardiomyocytes.

Aplicação

Endothelin-1 (ET-1) has been used to study the production and detection of H₂O₂ and production of NO in the presence of ET-1, for studying the effects of ET-1 on the eNOS/inducible NOS (iNOS)/caveolin-1 expression, NO production, isoform specific PKC translocation, and PKC activity in human saphenous vein endothelial cells (HSVECs).

Embalagem

10, 50 μg

0.1 mg

Ações bioquímicas/fisiológicas

Endothelin-1 (ET-1) is a vasoconstrictor, which is increased in plasma and explants of patients with uterine leiomyomas. Thus, uterine fibroids are linked to increased ET-1 levels and hypertension, which is worsened by hypoxic condtions. ET-1 RNAi and Endostar are capable of preventing the progression and invasiveness of PC-3 (prostate cancer) cells. The plasma levels of this peptide are increased in patients with atrial septal defect (ASD), and these levels might help in diagnosis of closure defects. The plasma level of ET-1 is also higher in patients with end-stage renal disease (ESRD), and this is linked to gradual worsening of pulmonary function tests.

Potent agonist for ET_A and ET_B endothelin receptors. Induces the production of hypoxia-inducible factor 1α, and, thus, the production of VEGF.

Outras notas

A synthetic peptide with the sequence of human and porcine Endothelin 1.

The biological source is human endothelin, which shares the same sequence

Lyophilized from 0.1% TFA in H2O

Safety Information

• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 3
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)