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FLAGIPT1

FLAG® Immunoprecipitation Kit

Sinônimo(s):

Anti-ddddk, Anti-dykddddk

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Tamanho/SKUDisponibilidadePreço
1 kit
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R$ 10.500,00

Sobre este item

NACRES:
NA.32
UNSPSC Code:
12352200

R$ 10.500,00


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Quality Segment

usage

 kit sufficient for 50 reactions

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

FLAG purification: suitable, immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3xFLAG™ peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este Item
CELLMM2F3040A2220
description

-

description

For Mammalian expression systems.

description

clone M1, purified immunoglobulin, buffered aqueous solution

description

purified immunoglobulin, buffered aqueous glycerol solution

technique(s)

immunoprecipitation (IP): suitable

technique(s)

protein extraction: suitable

technique(s)

western blot: 10 μg/mL

technique(s)

immunoprecipitation (IP): suitable, affinity chromatography: suitable

usage

 kit sufficient for 50 reactions

usage

-

usage

-

usage

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

analyte chemical class(es)

-

analyte chemical class(es)

-

analyte chemical class(es)

proteins


Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS & Preços

  • ANTI-FLAG M2 affinity gel 1 mL
    SDS

  • 3X FLAG peptide 1 mg
    SDS

Pictogramas

Exclamation mark

Classe de armazenamento

10 - Combustible liquids

O que está acontecendo?

WGK 3

Palavra de aviso

Danger

Códigos de risco

Declarações de precaução

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2



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