If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P7943
L-α-Phosphatidylethanolamine from egg yolk
Type III, ≥97% (TLC), lyophilized powder
Sinônimo(s):
1,2-Diacyl-sn-glycero-3-phosphoethanolamine, L-α-Cephalin
Selecione um tamanho
R$ 482,00
R$ 1.407,00
R$ 5.161,00
Sobre este item
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Nome do produto
L-α-Phosphatidylethanolamine from egg yolk, Type III, ≥97% (TLC), lyophilized powder
SMILES string
[P](=O)(OC[C@H](OC(=O)CCCCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCC)(OCCN)O
InChI key
NJGIRBISCGPRPF-KXQOOQHDSA-N
InChI
1S/C40H80NO8P/c1-3-5-7-9-11-13-15-17-18-19-20-21-23-25-27-29-31-33-40(43)49-38(37-48-50(44,45)47-35-34-41)36-46-39(42)32-30-28-26-24-22-16-14-12-10-8-6-4-2/h38H,3-37,41H2,1-2H3,(H,44,45)/t38-/m1/s1
biological source
egg yolk
type
Type III
assay
≥97% (TLC)
form
lyophilized powder
functional group
phospholipid
lipid type
phosphoglycerides
shipped in
ambient
storage temp.
−20°C
Quality Level
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1 of 4
Este Item | P3556 | P8318 | P4279 |
|---|---|---|---|
| functional group phospholipid | functional group phospholipid | functional group phospholipid | functional group phospholipid |
| assay ≥97% (TLC) | assay ≥99% (TLC) | assay ≥99% (TLC) | assay ≥99% (TLC) |
| biological source egg yolk | biological source egg yolk | biological source egg yolk | biological source egg yolk |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form solution |
Application
- as a component of lipid mixture for the synthesis of giant unilamellar vesicles (GUVs)[1]
- in ceramide phosphoethanolamine (CPE) synthase assay using crude sphingomyelin synthase-related protein from Hela cells[2]
- for the standard curve generation for the quantification of lipids in plantaris muscle homogenates from mice[3]
Disclaimer
General description
Packaging
Classe de armazenamento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocolos
(soybean), ≥97%; L-α-Phosphatidylethanolamine from egg yolk, Type III, ≥97% (TLC), lyophilized powder; L-α-Phosphatidylcholine, from egg yolk, Type XVI-E, ≥99% (TLC), lyophilized powder; Sphingomyelin, from chicken egg yolk, ≥95%
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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What solvents are used to dissolve phosphatidylethanolamine? What is the solubility? What solvent is.least toxic to eucaryotic cells?
1 answer-
This solubility is tested in CHCl3 at 25 mg/mL. Stock solutions in chloroform can be added to medium or buffer and allowed to stir prior to filtration. This allows the chloroform to evaporate off while keeping the Phosphatidylethanolamine in solution, thereby avoiding the risk of exposing cells to the solvent. An alternative solvent would be a mixture of chloroform and methanol, which would also be cytotoxic. Please see the sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P7943-BULKSLCN2430.pdfHelpful?
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