Non-Fat Dry Milk (NFDM) Solution for Blocking Western Blots
Non-fat dry milk (NFDM) blocking buffer is ideal for saturating excess protein-binding sites on membranes for Western blotting. The primary proteins in milk are casein and whey. Typically, 1-5% NFDM is sufficient for most Western blots. To make a 1% solution of NFDM, combine 1 g of NFDM and 100 mL of TBST.
This calculator enables the accurate NFDM solution preparation to make enough for a single experiment or for the entire lab. Input your desired concentration and volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.
Non-fat Dry Milk Buffer Recipe Calculator
| 5% in 100 mL Recipe | NFDM: % at Desired Volume: mL |
| 5 g of Non-fat dry milk powder | g Non-fat dry milk powder |
| in 100 mL of 1X TBST | in mL of 1X TBST |
OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS
Stock Solutions
- 4X SDS Sample Loading Buffer
- 10X Phosphate-Buffered Saline (PBS)
- 10X Tris-Buffered Saline (TBS) Stock Solution
- Stock Components for Western Blot Stripping Solutions
Wash & Blocking Buffers
- 1X Tris-Buffered Saline (TBS) Working Solution
- 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
- 1X PBS (Phosphate-Buffered Saline)
- 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
- Bovine Serum Albumin (BSA) Blocking Buffer
Stripping Solutions
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