MABF930M

Anti-TCR Vγ1.1 Antibody, clone 2.11

clone 2.11, from hamster(Armenian)

• Synonym(e): T-cell receptor gamma chain C region C10.5
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: hamster (Armenian)
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 2.11, monoclonal
• Speziesreaktivität: mouse
• Verpackung: antibody small pack of 25 μg
• Methode(n): flow cytometry: suitable; immunoprecipitation (IP): suitable
• Isotyp: IgGκ
• UniProt-Hinterlegungsnummer: P01853
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Tcrg (110067)

Beschreibung

Allgemeine Beschreibung

19.10 kDa calculated.

T cell receptor (TCR) is a single-pass membrane protein that associates with CD3 to form a CD3/TCR complex. It can be a heterodimer of either alpha and beta chains or gamma and delta chains. CD3/RCR complex plays major role in antigen recognition and activation of T cells. The V gamma 1 gene almost exclusively rearranges to the J gamma 4-C gamma 4 gene. V-gamma 1- J gamma 4-C gamma 4 expressing cells form a major population of gamma/delta T cells in thymus and peripheral lymphoid organs in adult mice. However, during early development they are present in lower numbers. This antibody (clone 2.11) recognizes the V gamma 1.1 chain of the heterodimeric gamma/delta T-cell receptor, but does react with TCR alpha/beta bearing cells.

Immunogen

Irradiated TCR gamma/delta expressing T3 .13.1 hybridoma cells.

Anwendung

Research Category
Entzündung & Immunologie

Anti-TCR V 1.1, clone 2.11, Cat. No. MABF930, is an armenian hamster monoclonal antibody that detects T-cell receptor gamma chain C region C10.5. and has been tested for use Flow Cytometry, and Immunoprecipitation.

Flow Cytometry Analysis: A representative lot detected TCR V gamma 1.1 in Flow Cytometry applications (Pereira, P., et. al. (1995). J Exp Med. 182(6):1921-30; Pereira, P., et. al. (2004). J Immunol. 173(5):3261-70).

Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected TCR V gamma 1.1 in Lymphocytes isolated from TcrdH2BeGFP reporter mice (Courtesy of Dr rer. nat. Inga Sandrock, Hannover Medical School, Germany).

Immunoprecipitation Analysis: A representative lot detected TCR V gamma 1.1 in Immunoprecipitation applications (Pereira, P., et. al. (1995). J Exp Med. 182(6):1921-30).

Biochem./physiol. Wirkung

Clone 2.11 detects T-cell receptor gamma chain C region C10.5 in lymphocytes.

Physikalische Form

Protein G purified

Format: Purified

Purified armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Angaben zur Herstellung

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable